Description |
1 online resource (x, 433 pages) : illustrations |
Series |
Methods in molecular biology, 1940-6029 ; 912 |
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Methods in molecular biology (Clifton, N.J.) ; v. 912. 1064-3745
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Contents |
Historical background of gamete and embryo culture / Jacques Cohen and Don Rieger -- Utility of animal models for human embryo culture development : rodents / Gary D. Smith -- Utility of animal models for human embryo culture development : domestic species / Rebecca L. Krisher -- Utility of animal models for human embryo culture : nonhuman primates / Calvin Simerly and Gerald Schatten -- Media composition : salts and osmolality / Jay M. Baltz -- Media composition : energy sources and metabolism / Deirdre Zander-Fox and Michelle Lane -- Media composition : amino acids and cellular homeostasis / Franchesca D. Houghton -- Media composition : macromolecules and embryo growth / Marius Meintjes -- Media composition : antioxidants/chelators and cellular function / Catherine M.H. Combelles and Margo L. Hennet -- Media composition : pH and buffers / Jason E. Swain -- Media composition : growth factors / Aparna Hegde and Barry Behr -- Culture systems : single step / Ronit Machtinger and Catherine Racowsky -- Culture systems : sequential / Patrick Quinn -- Culture systems : embryo co-culture / Yves J.R Ménézo, Edouard Servy, Anna Veiga, André Hazout, and Kay Elder -- Culture systems : low-oxygen culture / Borut Kovačič-- Culture systems : embryo density / Michael L. Reed -- Culture systems : air quality / Theodore Thomas -- Culture systems : mineral oil overlay / Dean E. Morbeck and Phoebe H. Leonard -- Culture systems : physiological and environmental factors that can affect the outcome of human ART / Don Rieger -- Culture systems : fluid dynamic embryo culture systems (microfluidics) / André Monteiro da Rocha and Gary D. Smith -- Human embryo culture media comparisons / Thomas B. Pool, John Schoolfield, and David Han -- Culture systems : embryo culture and monozygotic twinning / Amy E. Sparks -- Embryo culture and epigenetics / Brenna A. Market Velker, Michelle M. Denomme, and Mellissa R.W. Mann |
Summary |
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies |
Analysis |
embryokweek |
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embryo culture |
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celbiologie |
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cellular biology |
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mechatronica |
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mechatronics |
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protocollen |
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protocols |
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Biotechnology (General) |
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Biotechnologie (algemeen) |
Bibliography |
Includes bibliographical references and index |
Notes |
English |
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Print version record |
Subject |
Embryology -- Laboratory manuals
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Embryo Culture Techniques
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Embryology
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Human Anatomy & Physiology.
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Zoology.
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Health & Biological Sciences.
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Anatomy.
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Animal Anatomy & Embryology.
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Genre/Form |
Laboratory manuals
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Form |
Electronic book
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Author |
Smith, Gary D., 1964-
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Swain, Jason E.
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Pool, Thomas B.
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LC no. |
2012941744 |
ISBN |
9781617799716 |
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1617799718 |
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