| Description |
1 online resource (xv, 385 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 331 |
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Methods in molecular biology (Clifton, N.J.) ; v. 331
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| Contents |
Human embryonic stem cells : isolation, maintenance, and differentiation / Kursad Turksen and Tammy-Claire Troy -- Blastocyst culture for deriving human embryonic stem cells / Ariff Bongso -- Propagation of human embryonic stem cells on human feeder cells / Mark Richards and Ariff Bongso -- Derivation and maintenance of human embryonic stem cells / Michal Amit and Joseph Itskovitz-Eldor -- Isolation and characterization of human embryonic stem cells / Maisam Mitalipova and Gail Palmarini -- Routine culture and differentiation of human embryonic stem cells / Jim McWhir, Davina Wojtacha, and Alison Thomson -- Culture of human embryonic stem cells on human and mouse feeder cells / Gautam Dravid, Holly Hammond, and Linzhao Cheng |
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Maintenance of human embryonic stem cells in animal serum- and feeder layer-free culture conditions / Michal Amit and Joseph Itskovitz-Eldor -- Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor / Noboru Sato and Ali H. Brivanlou |
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Derivation of human feeders for prolonged support of human embryonic stem cells / Chui-Yee Fong and Ariff Bongso -- Transplantation of human embryonic stem cells to the chick embryo / Ronald S. Goldstein -- Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro / Melissa Carpenter [and others] -- In vitro differentiation of neural precursors from human embryonic stem cells / Xue-Jun Li and Su-Chun Zhang |
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Derivation and characterization of hematopoietic cells from human embryonic stem cells / Lisheng Wang [and others] -- Retroviral transduction of hematopoietic progenitors derived from human embryonic stem cells / Pablo Menendez [and others] -- Genetic manipulation of human embryonic stem cells by transfection / Rachel Eiges -- Designing, testing, and validating a microarray for stem cell characterization / Yongquan Luo [and others] -- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells / Noboru Sato and Ali H. Brivanlou -- Massively parallel signature sequencing / Daixing Zhou [and others] -- Confrontation cultures of embryonic stem cells with multicellular tumor spheroids to study tumor-induced angiogenesis / Maria Wartenberg [and others] |
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A novel experimental platform for investigating cancer growth and anti-cancer therapy in a human tissue microenvironment derived from human embryonic stem cells / Maty Tzukerman and Karl L. Skorecki -- The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells / Justin C. St. John [and others] |
| Summary |
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today |
| Analysis |
embryonale stamcellen |
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embryonic stem cells |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Embryonic stem cells -- Laboratory manuals
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Human embryo -- Laboratory manuals
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Stem cells.
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Cells.
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Research.
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Anatomy.
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Science.
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Physical sciences.
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Stem Cells
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Embryo Research
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Cells
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Investigative Techniques
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Biomedical Research
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Research
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Anatomy
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Science
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Natural Science Disciplines
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Disciplines and Occupations
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Animal Structures
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research (function)
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anatomy.
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sciences (philosophy)
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physical sciences.
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science (modern discipline)
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MEDICAL -- Research.
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Stem cells
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Science
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Research
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Physical sciences
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Cells
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Anatomy
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Embryonic stem cells
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Human embryo
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| Genre/Form |
CD-ROMs
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Laboratory manuals
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CD-ROMs.
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Turksen, Kursad.
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| ISBN |
9781597450461 |
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1597450464 |
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1280832010 |
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9781280832017 |
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9786610832019 |
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6610832013 |
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