Description |
1 online resource (xv, 449 pages) : illustrations (some color) |
Series |
Methods in molecular biology, 1940-6029 ; volume 1341 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 1341. 1064-3745
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Springer protocols (Series)
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Contents |
Maximizing clonal embryonic stem cell derivation by ERK pathway inhibition / Jennifer Nichols and Thorsten Boroviak -- Simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells / Keitaro Imaizumi [and others]. -- Resolving heterogeneity : fluorescence-activated cell sorting of dynamic cell populations from feeder-free mouse embryonic stem cell culture / Jurriaan Holzenspies, Gelo Dela Cruz, and Joshua M. Brickman -- Acquiring ground state pluripotency : switching mouse embryonic stem cells from serum/LlF medium to 2i/LlF medium / Matteo Tosolini and Alice Jouneau -- Generation of embryonic stem cells in rabbits / Marielle Afanassieff, Pierre Osteil, and Pierre Savatier -- Gene transfer into pluripotent stem cells via lentiviral transduction / Ortwin Naujok, Ulf Diekmann, and Matthias Elsner -- Imaging pluripotency : time-lapse Analysis of mouse embryonic stem cells / Anna Pezzarossa [and others] -- Expanding the utility of FUCCI reporters using FACS-based omics analysis / James Chappell, Ben Boward, and Stephen Dalton -- Selection of antibodies interfering with cell surface receptor signaling using embryonic stem cell differentiation / Anna N. Melidoni, Michael R. Dyson, and John McCafferty -- Delivering antisense morpholino oligonucleotides to target telomerase splice variants in human embryonic stem cells / Lida Radan [and others] -- Analysis of mRNA translation rate in mouse embryonic stem cells / Anisa B. Rahim and Leah A. Vardy -- Generation and purification of definitive endoderm cells generated from pluripotent stem cells / Ulf Diekmann and Ortwin Naujok -- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation / Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume -- Selective differentiation into hematopoietic and cardiac cells from pluripotent stem cells based on the expression of cell surface markers / Atsumasa Okada [and others] -- Methods for derivation of multipotent neural crest cells derived from human pluripotent stem cells / John Avery and Stephen Dalton -- From naive to primed pluripotency : in vitro conversion of mouse embryonic stem cells in Epiblast Stem Cells / Matteo Tosolini and Alice Jouneau -- In vitro differentiation of embryonic stem cells into hematopoietic lineage : towards erythroid progenitor's production / Iliana Fauzi, Nicki Panoskaltsis, and Athanasios Mantalaris -- Dopaminergic differentiation of human embryonic stem cells on PA6-derived adipocytes / M. Oktar Guloglu and Anna Larsen -- Intracellular calcium measurements for functional characterization of neuronal Phenotypes / Talita Glaser [and others] -- In vitro differentiation of pluripotent stem cells into functional b islets under 2D and 3D culture conditions and in vivo preclinical validation of 3D islets / Bipasha Bose and P Shenoy Sudheer -- Generation of corneal keratocytes from human embryonic stem cells / Andrew J. Hertsenberg and James L. Funderburgh -- Maintenance, transgene delivery, and pluripotency measurement of mouse embryonic stem cells / Tetsuya S. Tanaka -- Generation of a knockout mouse embryonic stem cell line using a paired CRISPR/Cas9 genome engineering tool / Rahel Wettstein, Maxime Bodak, and Constance Ciaudo -- Derivation of neural precursor cells from human embryonic stem cells for DNA methylomic analysis / Ivan Roubal, Sun Joo Park, and Yong Kim -- 26S and PA28-20S proteasome activity in cytosolic extracts from embryonic stem cells / Malin Hernebring -- Concise protocol for siRNA-mediated gene suppression in human embryonic stem cells / Peter F. Renz and Tobias A. Beyer -- Applying shear stress to pluripotent stem cells / Russell P. Wolfe [and others] -- Generating inner ear organoids from mouse embryonic stem cells / Emma Longworth-Mills, Karl R. Koehler, and Eri Hashino -- Differentiation of adipocytes in monolayer from mouse embryonic stem cells / Ixchelt Cuaranta-Monroy, Zoltan Simandi, and Laszlo Nagy -- Pancreatic differentiation from murine embryonic stem cells / Daisuke Sakano, Nobuaki Shiraki, and Shoen Kume -- Osteogenic differentiation from embryonic stem cells / Yanhong Yu, Carlos Pilquil, and Michal Opas -- Limbal stromal tissue specific stem cells and their differentiation potential to corneal epithelial cells / Kishore Reddy Katikireddy and Ula V. Jurkunas |
Summary |
This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to move very quickly, thus the kinds of studies continue to expand and diversify, and methodologies are continuously being refined and improved, which this book reflects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and fully updated, Embryonic Stem Cell Protocols, Third Edition serves as an ideal reference for researchers investigating this rich area of study |
Bibliography |
Includes bibliographical references and indexes |
Subject |
Stem cells -- Laboratory manuals
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Embryos -- Laboratory manuals
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Stem cells.
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Research.
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Cells.
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Anatomy.
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Science.
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Physical sciences.
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Embryo Research
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Models, Animal
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Embryonic Stem Cells
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Investigative Techniques
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Biomedical Research
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Stem Cells
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Research
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Cells
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Anatomy
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Science
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Natural Science Disciplines
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Disciplines and Occupations
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research (function)
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anatomy.
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sciences (philosophy)
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physical sciences.
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science (modern discipline)
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Science
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Research
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Physical sciences
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Cells
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Anatomy
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Embryos
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Stem cells
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Turksen, Kursad, editor
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ISBN |
9781493929542 |
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1493929542 |
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1493929534 |
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9781493929535 |
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