Description |
1 online resource (1 volume) : illustrations |
Series |
Methods in molecular biology ; v. 330, pt. 2 |
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Methods in molecular biology (Clifton, N.J.) ; volume 330, pt. 2
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Contents |
Neural stem sphere method: Induction of neural stem cells and neurons by astrocyte-derived factors in embryonic stem cells in vitro / T. Nakayama and N. Inoue -- Generation and characterization of oligodendrocytes from lineage-selectable embryonic stem cells in vitro / N. Billon, C. Jolicoeur and M. Raff -- Derivation and characterization of neural cells from embryonic stem cells using nestin enhancer / N. Lenka -- Optimized neuronal differentiation of murine embryonic stem cells: Role of cell density / M.T. Lorincz -- Generation of inner ear cell types from embryonic stem cells / M.N. Rivolta, H. Li and S. Heller -- Derivation of epidermal colony-forming progenitors from embryonic stem cell cultures / T.C. Troy and K. Turksen -- Directing epidermal fate selection by a novel co-culture system / T.C. Troy and K. Turksen -- In vitro generation of T lymphocytes from embryonic stem cells / R.F. de Pooter, T.M. Schmitt and J.C. Zuniga-Pflucker -- The role of hex in hemangioblast and hematopoietic development / R.J. Chan, R. Hromas and M.C. Yoder -- Generation of osteoblasts and chondrocytes from embryonic stem cells / J. Kawaguchi -- Analysis of embryonic stem cell-derived osteogenic cultures / N.L. Woll and S.K. Bronson -- Generation of chondrocytes from embryonic stem cells / J.S. Khillan -- Derivation and characterization of chondrocytes from embryonic stem cells in vitro / J. Kramer, G. Hargus and J. Rohwedel -- Generation and characterization of cardiomyocytes under serum-free conditions / C. Gissel [and others] -- Analysis of arrhythmic potential of embryonic stem cell-derived cardiomyocytes / L.L. Shang, S.C. Dudley, Jr. and A.E. Pfahnl -- Derivation and characterization of alveolar epithelial cells from murine embryonic stem cells in vitro / A. Samadikuchaksaraei and A.E. Bishop -- Derivation and characterization of thyrocyte-like cells from embryonic stem cells in vitro / R.Y. Lin and T.F. Davies -- Derivation and characterization of gut-like structures from embryonic stem cells / T. Yamada and Y. Nakajima -- Formation of gut-like structures in vitro from mouse embryonic stem cells / S. Torihashi -- In vitro derivation and expansion of endothelial cells from embryonic stem cells / K.E. McCloskey, S.L. Stice and R.M. Nerem -- Differentiation of mouse embryonic stem cells into endothelial cells: Genetic selection and potential use in vivo / C. Gimond, S. Marchetti and G. Pages -- Integrins and vascular development in differentiated embryonic stem cells in vitro / S.E. Francis -- TGF-[beta] signaling in embryonic stem cell-derived endothelial cells / T. Watabe [and others] -- The role of the adapter protein SHB in embryonic stem cell differentiation into the pancreatic [beta]-cell and endothelial lineages / J. Saldeen [and others] -- In vitro differentiation of embryonic stem cells into the pancreatic lineage / P. Blyszczuk and A.M. Wobus -- Derivation and characterization of hepatocytes from embryonic stem cells in vitro / S. Ishizaka [and others] -- Differentiation of embryonic stem cells to retinal cells in vitro / X. Zhao, J. Liu and I. Ahmad -- Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro / M. Takahashi and M. Haruta -- Differentiation of rhesus monkey embryonic stem cells in three-dimensional collagen matrix / S.S. Chen [and others] |
Summary |
Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. A companion first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, focuses on ES cells recently isolated from different nonhuman species, to provide a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area |
Bibliography |
Includes bibliographical references and index |
Notes |
Print version record |
Subject |
Stem cells -- Laboratory manuals
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Embryos -- Laboratory manuals
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Stem cells.
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Stem Cells
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Embryo Research
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Models, Animal
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MEDICAL -- Research.
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Embryos
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Stem cells
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Turksen, Kursad
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ISBN |
1597450367 |
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9781597450362 |
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