Title Human embryogenesis : methods and protocols / edited by Julie Lafond and Cathy Vaillancourt

Published New York : Humana Press, ©2009

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Description 1 online resource (x, 312 pages) : illustrations Series Springer protocols Methods in molecular biology, 1064-3745 ; 550 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 550. Contents Human embryogenesis: overview / C. Vaillancourt and J. Lafond -- Differential gene expression in the uterus and blastocyst during the reactivation of embryo development in a model of delayed implantation / P.L. Lefevre and B.D. Murphy -- Coculture of decidua and trophoblast to study proliferation and invasion / M. Cohen and P. Bischof -- Isolation and culture of term human cytotrophoblast cells and in vitro methods for studying human cytotrophoblast cells' calcium uptake / F. Le Bellego, C. Vaillancourt and J. Lafond -- Endocrinology and cell signaling in human villous trophoblast / C. Mounier [and others] -- Gestation-induced uterine vascular remodeling / P.A. Scott [and others] -- Placental and fetal steroidogenesis / J.T. Sanderson -- Current methods in investigating the development of the female reproductive system / P.J. Devine, P.B. Hoyer and A.F. Keating -- A revised and improved method for the isolation of seminiferous tubule-enriched fractions that preserves the phosphorylated and glycosylated forms of proteins / C.D. Akpovi and R. Marc Pelletier -- A novel technical approach for the measurement of individual ACAT-1 and ACAT-2 enzymatic activity in the testis / L. Chen, J. Lafond and R.M. Pelletier -- Genetic dissection of Caenorhabditis elegans embryogenesis using RNA interference and flow cytometry / E.L. Boulier and S. Jenna -- Immune system: maturation of myeloid cells / J. Ennaciri and D. Girard -- Functional development of human fetal gastrointestinal tract / E. Levy [and others] -- Renal and cardiac Na+-K +-ATPase and aconitase in a rat model of fetal programming / R. Gaudet and M. Brochu -- Assessment of embryotoxicity using mouse embryo culture / L.M. Winn and E.W. Tung -- Fertilization in vitro / Y. Bing and R.J. Ouellette -- Animal cloning by somatic cell nuclear transfer / L.C. Smith and J.G. Yoo -- The human embryo: ethical and legal aspects / B.M. Knoppers, S. Bordet and R. Isasi Summary Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis Analysis embryos embryogenese embryogenesis embryokloneren embryo cloning embryonale ontwikkeling embryonic development foetale groei fetal growth laboratoriummethoden laboratory methods protocollen protocols Cellular Biology Celbiologie Bibliography Includes bibliographical references and index Notes English Print version record Subject Embryology, Human -- Laboratory manuals Fertilization in vitro -- Laboratory manuals Cloning -- Laboratory manuals Embryology, Experimental -- Moral and ethical aspects -- Laboratory manuals Embryology. Genetics -- Technique. Fertilization in vitro, Human. Fertilization in vitro. Methodology. Human reproductive technology. Biology. Morphogenesis. Reproductive technology. Life sciences. Physical sciences. Therapeutics. Genetics Embryonic Development Genetic Techniques Fertilization in Vitro Laboratory Manuals Methods Reproductive Techniques, Assisted Embryonic and Fetal Development Investigative Techniques Biology Publication Formats Morphogenesis Reproductive Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Reproduction Biological Science Disciplines Publication Characteristics Natural Science Disciplines Reproductive Physiological Phenomena Growth and Development Therapeutics Disciplines and Occupations Physiological Phenomena Reproductive and Urinary Physiological Phenomena Phenomena and Processes methodology. biology. biological sciences. physical sciences. treating (health care function) Therapeutics Reproductive technology Physical sciences Morphogenesis Methodology Life sciences Human reproductive technology Genetics -- Technique Fertilization in vitro, Human Embryology Biology Cloning Embryology, Human Fertilization in vitro Développement embryonnaire -- génétique -- Cahiers de laboratoire. Techniques génétiques -- Cahiers de laboratoire. Embryonalentwicklung Labortechnik Embryologie humaine -- Manuels de laboratoire. Fécondation in vitro -- Manuels de laboratoire. Clonage -- Manuels de laboratoire. Embryologie expérimentale -- Aspect moral -- Manuels de laboratoire. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Lafond, Julie Vaillancourt, Cathy ISBN 9781603270090 1603270094

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