Title Septic shock methods and protocols / edited by Thomas J. Evans

Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (x, 208 pages) : illustrations Series Methods in molecular medicine ; 36 Methods in molecular medicine ; 36. Contents Assay of endotoxin by limulus amebocyte lysate / P.A. Ketchum and T.J. Novitsky-Preparation of endotoxin from pathogenic gram-negative bacteria / A. Shnyra, M. Luchi and D.C. Morrison-Assay of anti-endotoxin antibodies / L. Brade-Isolation of the bactericidal/permeability-increasing protein from polymorphonuclear leukocytes by reversible binding to target bacteria / J. Weiss-Purification of lipopolysaccharide binding protein / D. Heumann-Purification of streptococcal pyrogenic exotoxin a / M. Roggiani and P.M. Schlievert-Assay for superantigens / S. Sriskandan-Bioassay for tumor necrosis factors-alpha and beta / T.J. Evans-Assay of soluble tumor necrosis factor receptors / M.G. Bouma and W.A. Buurman-Whole-blood assays for cytokine production / D.G. Remick, D.E. Newcomb and J.S. Friedland-Nitric oxide synthase inhibitors / D.K. Siriwardena, H. Tagori and C. Thiemermann-In situ detection of nitric oxide / T. Malinski-Immunochemical detection of nitric oxide synthase in human tissue / L.D. Buttery and J.M. Polak-Anti-nitrotyrosine antibodies for immunohistochemistry / L. Viera, Y.Z. Ye and J.S. Beckman-Detection of peroxynitrite in biological fluids / S. Malcolm [and others]-Myocardial cells in culture to study effects of cytokines / K.A. Krown and R.A. Sabbadini-Culture of primary human bronchial epithelial cells / J. Picot-Primary culture of human proximal renal tubular epithelial cells / P.A. Glynne Summary In Septic Shock Methods and Protocols, an interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of whole animals-these experienced laboratorians detail the most important techniques for studying sepsis involving endotoxins, toxins derived from gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and the use of primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells. Multidisciplinary and state-of-the-art, Septic Shock Methods and Protocols offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection. Its readily reproducible methods will allow both newcomers and those with more experience to apply today's powerful techniques successfully in their work Bibliography Includes bibliographical references and index Notes Assay of endotoxin by limulus amebocyte lysate / P.A. Ketchum and T.J. Novitsky-Preparation of endotoxin from pathogenic gram-negative bacteria / A. Shnyra, M. Luchi and D.C. Morrison-Assay of anti-endotoxin antibodies / L. Brade-Isolation of the bactericidal/permeability-increasing protein from polymorphonuclear leukocytes by reversible binding to target bacteria / J. Weiss-Purification of lipopolysaccharide binding protein / D. Heumann-Purification of streptococcal pyrogenic exotoxin a / M. Roggiani and P.M. Schlievert-Assay for superantigens / S. Sriskandan-Bioassay for tumor necrosis factors-alpha and beta / T.J. Evans-Assay of soluble tumor necrosis factor receptors / M.G. Bouma and W.A. Buurman-Whole-blood assays for cytokine production / D.G. Remick, D.E. Newcomb and J.S. Friedland-Nitric oxide synthase inhibitors / D.K. Siriwardena, H. Tagori and C. Thiemermann-In situ detection of nitric oxide / T. Malinski-Immunochemical detection of nitric oxide synthase in human tissue / L.D. Buttery and J.M. Polak-Anti-nitrotyrosine antibodies for immunohistochemistry / L. Viera, Y.Z. Ye and J.S. Beckman-Detection of peroxynitrite in biological fluids / S. Malcolm [and others]-Myocardial cells in culture to study effects of cytokines / K.A. Krown and R.A. Sabbadini-Culture of primary human bronchial epithelial cells / J. Picot-Primary culture of human proximal renal tubular epithelial cells / P.A. Glynne Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Print version record Subject Septic shock -- Research -- Methodology Endotoxins -- Research -- Methodology Cytokines -- Research -- Methodology Septicemia. Bacterial toxins. Free radicals (Chemistry) Nitrogen oxides. Cultures (Biology) Medical laboratory technology. Oxides. Toxins. Nitrogen compounds. Inorganic compounds. Infection. Shock. Proteins. Biological products. Biologicals. Inflammation. Diseases. Septic shock. Cytokines. Endotoxins. Nitric oxide. Methodology. Sepsis Investigative Techniques Bacterial Toxins Free Radicals Nitrogen Oxides Reactive Nitrogen Species Intercellular Signaling Peptides and Proteins Systemic Inflammatory Response Syndrome Culture Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Clinical Laboratory Techniques Oxides Toxins, Biological Nitrogen Compounds Inorganic Chemicals Infections Shock Proteins Peptides Biological Factors Chemicals and Drugs Bacterial Infections and Mycoses Amino Acids, Peptides, and Proteins Oxygen Compounds Inflammation Pathologic Processes Pathological Conditions, Signs and Symptoms Disease Shock, Septic Cell Culture Techniques Cytokines Endotoxins Nitric Oxide Methods Biological Products nitrogen compounds. protein. disease. illness. methodology. MEDICAL -- Evidence-Based Medicine. HEALTH & FITNESS -- Diseases -- General. MEDICAL -- Diseases. MEDICAL -- Internal Medicine. MEDICAL -- Clinical Medicine. Toxins Shock Septicemia Septic shock Proteins Oxides Nitrogen oxides Nitrogen compounds Nitric oxide Methodology Medical laboratory technology Inorganic compounds Inflammation Infection Free radicals (Chemistry) Endotoxins Diseases Cytokines Cultures (Biology) Biologicals Biological products Bacterial toxins Cytokines -- Research -- Methodology Wetenschappelijke technieken. Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Evans, Thomas J., 1959- LC no. 99029663 ISBN 9781592592166 1592592163 0896037304 9780896037304 0585355665 9780585355665 1280830239 9781280830235 9786610830237 6610830231

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