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Title Septic shock methods and protocols / edited by Thomas J. Evans
Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (x, 208 pages) : illustrations
Series Methods in molecular medicine ; 36
Methods in molecular medicine ; 36.
Contents Assay of endotoxin by limulus amebocyte lysate / P.A. Ketchum and T.J. Novitsky-Preparation of endotoxin from pathogenic gram-negative bacteria / A. Shnyra, M. Luchi and D.C. Morrison-Assay of anti-endotoxin antibodies / L. Brade-Isolation of the bactericidal/permeability-increasing protein from polymorphonuclear leukocytes by reversible binding to target bacteria / J. Weiss-Purification of lipopolysaccharide binding protein / D. Heumann-Purification of streptococcal pyrogenic exotoxin a / M. Roggiani and P.M. Schlievert-Assay for superantigens / S. Sriskandan-Bioassay for tumor necrosis factors-alpha and beta / T.J. Evans-Assay of soluble tumor necrosis factor receptors / M.G. Bouma and W.A. Buurman-Whole-blood assays for cytokine production / D.G. Remick, D.E. Newcomb and J.S. Friedland-Nitric oxide synthase inhibitors / D.K. Siriwardena, H. Tagori and C. Thiemermann-In situ detection of nitric oxide / T. Malinski-Immunochemical detection of nitric oxide synthase in human tissue / L.D. Buttery and J.M. Polak-Anti-nitrotyrosine antibodies for immunohistochemistry / L. Viera, Y.Z. Ye and J.S. Beckman-Detection of peroxynitrite in biological fluids / S. Malcolm [and others]-Myocardial cells in culture to study effects of cytokines / K.A. Krown and R.A. Sabbadini-Culture of primary human bronchial epithelial cells / J. Picot-Primary culture of human proximal renal tubular epithelial cells / P.A. Glynne
Summary In Septic Shock Methods and Protocols, an interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of whole animals-these experienced laboratorians detail the most important techniques for studying sepsis involving endotoxins, toxins derived from gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and the use of primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells. Multidisciplinary and state-of-the-art, Septic Shock Methods and Protocols offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection. Its readily reproducible methods will allow both newcomers and those with more experience to apply today's powerful techniques successfully in their work
Bibliography Includes bibliographical references and index
Notes Assay of endotoxin by limulus amebocyte lysate / P.A. Ketchum and T.J. Novitsky-Preparation of endotoxin from pathogenic gram-negative bacteria / A. Shnyra, M. Luchi and D.C. Morrison-Assay of anti-endotoxin antibodies / L. Brade-Isolation of the bactericidal/permeability-increasing protein from polymorphonuclear leukocytes by reversible binding to target bacteria / J. Weiss-Purification of lipopolysaccharide binding protein / D. Heumann-Purification of streptococcal pyrogenic exotoxin a / M. Roggiani and P.M. Schlievert-Assay for superantigens / S. Sriskandan-Bioassay for tumor necrosis factors-alpha and beta / T.J. Evans-Assay of soluble tumor necrosis factor receptors / M.G. Bouma and W.A. Buurman-Whole-blood assays for cytokine production / D.G. Remick, D.E. Newcomb and J.S. Friedland-Nitric oxide synthase inhibitors / D.K. Siriwardena, H. Tagori and C. Thiemermann-In situ detection of nitric oxide / T. Malinski-Immunochemical detection of nitric oxide synthase in human tissue / L.D. Buttery and J.M. Polak-Anti-nitrotyrosine antibodies for immunohistochemistry / L. Viera, Y.Z. Ye and J.S. Beckman-Detection of peroxynitrite in biological fluids / S. Malcolm [and others]-Myocardial cells in culture to study effects of cytokines / K.A. Krown and R.A. Sabbadini-Culture of primary human bronchial epithelial cells / J. Picot-Primary culture of human proximal renal tubular epithelial cells / P.A. Glynne
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
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Subject Septic shock -- Research -- Methodology
Endotoxins -- Research -- Methodology
Cytokines -- Research -- Methodology
Septicemia.
Bacterial toxins.
Free radicals (Chemistry)
Nitrogen oxides.
Cultures (Biology)
Medical laboratory technology.
Oxides.
Toxins.
Nitrogen compounds.
Inorganic compounds.
Infection.
Shock.
Proteins.
Biological products.
Biologicals.
Inflammation.
Diseases.
Septic shock.
Cytokines.
Endotoxins.
Nitric oxide.
Methodology.
Sepsis
Investigative Techniques
Bacterial Toxins
Free Radicals
Nitrogen Oxides
Reactive Nitrogen Species
Intercellular Signaling Peptides and Proteins
Systemic Inflammatory Response Syndrome
Culture Techniques
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Clinical Laboratory Techniques
Oxides
Toxins, Biological
Nitrogen Compounds
Inorganic Chemicals
Infections
Shock
Proteins
Peptides
Biological Factors
Chemicals and Drugs
Bacterial Infections and Mycoses
Amino Acids, Peptides, and Proteins
Oxygen Compounds
Inflammation
Pathologic Processes
Pathological Conditions, Signs and Symptoms
Disease
Shock, Septic
Cell Culture Techniques
Cytokines
Endotoxins
Nitric Oxide
Methods
Biological Products
nitrogen compounds.
protein.
disease.
illness.
methodology.
MEDICAL -- Evidence-Based Medicine.
HEALTH & FITNESS -- Diseases -- General.
MEDICAL -- Diseases.
MEDICAL -- Internal Medicine.
MEDICAL -- Clinical Medicine.
Toxins
Shock
Septicemia
Septic shock
Proteins
Oxides
Nitrogen oxides
Nitrogen compounds
Nitric oxide
Methodology
Medical laboratory technology
Inorganic compounds
Inflammation
Infection
Free radicals (Chemistry)
Endotoxins
Diseases
Cytokines
Cultures (Biology)
Biologicals
Biological products
Bacterial toxins
Cytokines -- Research -- Methodology
Wetenschappelijke technieken.
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Evans, Thomas J., 1959-
LC no. 99029663
ISBN 9781592592166
1592592163
0896037304
9780896037304
0585355665
9780585355665
1280830239
9781280830235
9786610830237
6610830231