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Title Molecular epidemiology of microorganisms : methods and protocols / edited by Dominique A. Caugant
Published New York : Humana Press, ©2009


Description 1 online resource (xi, 321 pages) : illustrations
Series Springer protocols
Methods in molecular biology, 1064-3745 ; 551
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 551.
Contents Microbial molecular epidemiology : an overview / M. Tibayrenc -- Multilocus enzyme electrophoresis for parasites and other pathogens / M. Tibayrenc -- Plasmid replicon typing / T.J. Johnson and L.K. Nolan -- The application of randomly amplified DNA analysis in the molecular epidemiology of microorganisms / A. Belkum, E. Pelt-Verkuil and J.P. Hays -- Use of repetitive element palindromic PCR (rep-PCR) for the epidemiologic discrimination of foodborne pathogens / K.L. Hiett and B.S. Seal -- Pulsed-field gel electrophoresis for molecular epidemiology of food pathogens / T.M. Peters -- Molecular genotyping of microbes by multilocus PCR and mass spectrometry : a new tool for hospital infection control and public health surveillance / D.J. Ecker [and others] -- Amplified fragment-length polymorphism analysis / N.K. Fry, P.H. Savelkoul and P. Visca -- Clustered regularly interspaced short palindromic repeats (CRISPRs) for the genotyping of bacterial pathogens / I. Grissa, G. Vergnaud and C. Pourcel -- Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex / J.R. Driscoll -- Multilocus sequence typing / A.B. Ibarz Pavon and M.C. Maiden -- Multiple locus variable number of tandem repeats analysis / G. Vergnaud and C. Pourcel -- Comparison of molecular typing methods applied to Clostridium difficile / E.J. Kuijper, R.J. van den Berg and J.S. Brazier -- Genotyping of Mycobacterium tuberculosis clinical isolates using IS6110-based restriction fragment length polymorphism analysis / P. Bifani [and others] -- spa typing for epidemiological surveillance of Staphylococcus aureus / M. Hallin, A.W. Friedrich and M.J. Struelens -- Sequencing of viral genes / C. Holm-Hansen and K. Vainio -- Full sequencing of viral genomes : Practical strategies used for the amplification and characterization of foot-and-mouth disease virus / E.M. Cottam [and others] -- Bacterial genome sequencing / H. Tettelin and T. Feldblyum -- DNA microarray for molecular epidemiology of Salmonella / S. Huehn and B. Malorny -- Methods for data analysis / W.P. Hanage and D.M. Aanensen -- Internet-based sequence-typing databases for bacterial molecular epidemiology / K.A. Jolley
Summary The development of molecular genotyping methods has revolutionized the possibility for classification of microorganisms at the sub-species level. This investigation of species diversity is crucial for deciding the molecular relatedness of isolates for epidemiological studies. In Molecular Epidemiology of Microorganisms: Methods and Protocols, readers will find readily reproducible methods for determining the molecular epidemiology of microorganisms, all of which provide effective discrimination of unrelated strains. This volume covers a wide range of techniques which can be easily applied to the investigation of transmissible diseases, directing researchers towards the most effective methods based on the particular information to be obtained. Describing both traditional and novel techniques, expert researchers present a series of methods-based chapters with applications to some of the most important microbes. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Molecular Epidemiology of Microorganisms: Methods and Protocols provides state-of-the-art techniques which will prove to be critical in unraveling the routes of spread of pathogens for humans, animals, and plants
Analysis moleculaire epidemiologie
molecular epidemiology
Microbiology (General)
Microbiologie (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Molecular epidemiology -- Laboratory manuals
Pathogenic bacteria.
Molecular Epidemiology -- methods
Molecular epidemiology
Pathogenic bacteria
Épidémiologie moléculaire.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Caugant, Dominique A
ISBN 9781603279994