Title Epithelial cell culture protocols / edited by Clare Wise

Published Totowa, NJ : Humana Press, 2002

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Description 1 online resource (xiv, 405 pages) : illustrations Series Methods in molecular biology ; 188 Methods in molecular biology (Clifton, N.J.) ; v. 188 Contents Epithelial Cell Culture Protocols -- Preface -- Contents -- Contributors -- 1 Human Lens Epithelial Cell Culture -- 2 Human Airway Epithelial Cell Culture -- 3 Rat Gastric Mucosal Epithelial Cell Culture -- 4 Thymic Epithelial Cell Culture -- 5 Bile Duct Epithelial Cell Culture -- 6 Liver Epithelial Cell Culture -- 7 Pulmonary Epithelial Cell Culture -- 8 Prostate Epithelial Cell Isolation and Culture -- 9 A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier 10 The Bloodâ€?CSF Barrier in Culture Development of a Primary Culture and Transepithelial Transport Model from Choroidal Epithelial Cells11 An In Vitro Model of Differentiated Human Airway Epithelia Methods for Establishing Primary Cultures -- 12 Primary Mouse Keratinocyte Culture -- 13 Analyzing Apoptosis in Cultured Epithelial Cells -- 14 Keratins as Markers of Epithelial Cells -- 15 Epithelial Cell Integrins Joachim Rychly 1. -- 16 Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes 17 Clinical Application of Autologous Cultured Epithelia for the Treatment18 Transplantation of Cultured Epithelial Cells -- 19 An Outgrowth Culture System of Normal Human Epithelium An In Vitro Model to Study the Coordination of Cellular Migration and Proliferation -- 20 Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract -- 21 Applications of Epithelial Cell Culture in Studies of Drug Transport -- 22 X-Ray Microanalysis of Epithelial Cells in Culture -- 23 ENU Mutagenesis of Rat Mammary Epithelial Cells -- 24 ENU-Induced Ovarian Cancer 25 Measurement of Membrane Capacitance in Epithelial Monolayers26 Assessing Epithelial Cell Confluence by Spectroscopy Simon A. Lewis 1. -- 27 Co-Cultivation of Liver Epithelial Cells with Hepatocytes -- 28 Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth -- 29 Establishing Epithelialâ€?Immune Cell Co-Cultures Effects on Epithelial Ion Transport and Permeability -- 30 Explant Cultures of Embryonic Epithelium Analysis of Mesenchymal Signals -- 31 Bacterial Interactions with Host Epithelium In Vitro -- Index Summary Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Epithelial cells -- Laboratory manuals Epithelium -- Cultures and culture media -- Laboratory manuals Cell culture -- Laboratory manuals Epithelial cells. Epithelial Cells Epithelium -- anatomy & histology Cell Culture Techniques MEDICAL -- Histology. Cell culture Epithelial cells Epithelium -- Cultures and culture media Cellules en culture. Cellules épithéliales. Epitheel. Celkweek. Wetenschappelijke technieken. Cellules épithéliales. Épithélium -- Cultures et milieux de culture. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Wise, Clare LC no. 2001039554 ISBN 9781592591855 0896038939 9780896038936 0585403317 9780585403311 159259185X 9786610821747 6610821747 1280821744 9781280821745

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