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Title DNA repair protocols : eukaryotic systems / edited by Daryl S. Henderson
Published Totowa, N.J. : Humana Press, ©1999


Description 1 online resource (xix, 641 pages) : illustrations
Series Methods in molecular biology ; v. 113
Methods in molecular biology (Clifton, N.J.) ; v. 113
Contents Isolation of DNA structure-dependent checkpoint mutants in S. Pombe / R.G. Martinho and A.M. Carr -- Isolating mutants of the nematode Caenorhabditis elegans that are hypersensitive to DNA-damaging agents / P.S. Hartman and N. Ishii -- Isolating DNA repair mutants of drosophila melanogaster / D.S. Henderson -- Generation, identification, and characterization of repair-defective mutants of Arabidopsis / A. Britt and C.Z. Jiang -- Screening for [gamma]-ray hypersensitive mutants of Arabidopsis / C.S. Davies -- Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / M.Z. Zdzienicka -- Strategies for cloning mammalian DNA repair genes / L.H. Thompson -- Novel complementation assays for DNA repair-deficient cells. Transient and stable expression of DNA repair genes / L. Zeng, A. Sarasin and M. Mezzina -- The use of electrophoretic mobility shift assays to study DNA repair / B.J. Hwang, V. Smider and G. Chu -- Mismatch repair assay / S.E. Corrette-Bennett and R.S. Lahue -- Measurement of activities of cyclobutane-pyrimidine-dimer and (6-4)-photoproduct photolyases / J.B. Hays and P. Hoffman -- A dot blot immunoassay for UV photoproducts / S. McCready -- Measurement of UV radiation-induced DNA damage using specific antibodies / A.E. Stapleton -- Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / D.L. Mitchell -- Monitoring removal of cyclobutane pyrimidine dimers in Arabidopsis / J.B. Hays and Q. Pang -- DNA damage quantitation by alkaline gel electrophoresis / B.M. Sutherland, P.V. Bennett and J.C. Sutherland -- The comet assay (single-cell gel test). A sensitive genotoxicity test for the detection of DNA damage and repair / G. Speit and A. Hartmann -- Measuring the formation and repair of UV photoproducts by ligation-mediated PCR / G.P. Pfeifer and R. Dammann -- PCR-based assays for strand-specific measurement of DNA damage and repair. I. Strand-specific quantitative PCR / K.A. Grimaldi, J.P. Bingham and J.A. Hartley -- PCR-based assays for strand-specific measurement of DNA damage and repair. Ii. Single-strand ligation-PCR / K.A. Grimaldi, S.R. McAdam and J.A. Hartley -- Gene-specific and mitochondrial repair of oxidative DNA damage / R.M. Anson and V.A. Bohr -- Characterization of DNA strand cleavage by enzymes that act at abasic sites in DNA / W.A. Deutsch and A. Yacoub -- Base excision repair assay using Xenopus laevis oocyte extracts / Y. Matsumoto -- In vitro base excision repair assay using mammalian cell extracts / G. Frosina [and others] -- Nucleotide excision repair in saccharomyces cerevisiae whole-cell extracts / J.M. Wong, Z. He and C.J. Ingles -- In vitro excision repair assay in schizosaccharomyces pombe / B. Salles and P. Calsou -- Nucleotide excision repair assay in drosophila melanogaster using established cell lines / K. Kohno and T. Shimamoto -- Nucleotide excision repair in nuclear extracts from Xenopus oocytes / E.J. Ackerman [and others] -- Assay for nucleotide excision repair protein activity using fractionated cell extracts and UV-damaged plasmid DNA / M. Biggerstaff and R.D. Wood -- Dual-incision assays for nucleotide excision repair using DNA with a lesion at a specific site / M.K. Shivji [and others] -- In vitro chemiluminescence assay to measure excision repair in cell extracts / B. Salles and C. Provot -- Physical monitoring of ho-induced homologous recombination / A. Holmes and J.E. Haber -- Use of P element transposons to study DNA double-strand break repair in drosophila melanogaster / D.S. Henderson -- Analyzing double-strand repair events in drosophila / G.B. Gloor, T. Dray and K. Keeler -- Expression of i-sce i in drosophila to induce DNA double-strand breaks / V.A. Mogila, Y. Bellaiche and N. Perrimon -- Use of i-sce i to induce DNA double-strand breaks in nicotiana / H. Puchta -- Chromosomal double-strand breaks introduced in mammalian cells by expression of i-sce i endonuclease / C. Richardson, B. Elliott and M. Jasin -- Induction of DNA double-strand breaks by electroporation of restriction enzymes into mammalian cells / J.P. Carney and W.F. Morgan -- In vitro rejoining of double-strand breaks in genomic DNA / G. Iliakis and N. Cheong -- Extrachromosomal assay for DNA double-strand break repair / F. Liang and M. Jasin -- Use of gene targeting to study recombination in mammalian DNA repair mutants / R.S. Nairn and G.M. Adair -- Measurement of low-frequency DNA breaks using nucleoid flow cytometry / A.T. Vaughan, S. Walter and A.E. Milner -- Live analysis of the division cycles in x-irradiated drosophila embryos / J. Cunniff, J. Blethrow and W. Sullivan -- Inhibition of DNA synthesis by ionizing radiation / N.G. Jaspers and M.Z. Zdzienicka -- Analysis of inhibition of DNA replication in irradiated cells using the sv40-based in vitro assay of DNA replication / G. Iliakis, Y. Wang and H.Y. Wang -- Assays of bypass replication of genotoxic lesions in mammalian disease and mutant cell-free extracts / D.L. Svoboda and J.M. Vos -- Detection of chromatin-bound pcna in cultured cells following exposure to DNA-damaging agents / M. Miura and T. Sasaki -- Induction of p53 protein as a marker for ionizing radiation exposure in vivo / D.E. MacCallum and T.R. Hupp -- Activation of p53 protein function in response to cellular irradiation / J.P. Blaydes, A. Sparks and T.R. Hupp -- Selective extraction of fragmented DNA from apoptotic cells for analysis by gel electrophoresis and identification of apoptotic cells by flow cytometry / Z. Darzynkiewicz and G. Juan -- Detection of DNA strand breakage in the analysis of apoptosis and cell proliferation by flow and laser scanning cytometry / Z. Darzynkiewicz, X. Li and E. Bedner -- Immunoassay for single-stranded DNA in apoptotic cells / O.S. Frankfurt
Summary In DNA Repair Protocols: Eukaryotic Systems, Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants. DNA Repair Protocols: Eukaryotic Systems offers the most comprehensive collection of DNA repair protocols available, all step-by-step, optimized, and eminently reproducible with tips on troubleshooting and avoiding pitfalls. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches
Bibliography Includes bibliographical references and index
Notes English
Print version record
In Springer Protocols
Subject DNA repair -- Laboratory manuals
DNA repair.
DNA Repair
Eukaryotic Cells -- cytology
SCIENCE -- Life Sciences -- Genetics & Genomics.
DNA repair
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Henderson, Daryl S.
ISBN 9781592596751