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Title Cell cycle control : mechanisms and protocols / edited by Tim Humphrey, Gavin Brooks
Published Totowa, N.J. : Humana Press, ©2005


Description 1 online resource (xii, 402 pages) : illustrations
Series Methods in molecular biology ; 296
Methods in molecular biology (Clifton, N.J.) ; v. 296
Contents Cell cycle molecules and mechanisms of the budding and fission yeasts / Tim Humphrey and Amanda Pearce -- The plant cell cycle / John H. Doonan -- The C. elegans cell cycle / Sander van den Heuvel -- Developmental control of growth and cell cycle progression in drosophila / Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio -- The xenopus cell cycle / Anna Philpott and P. Renee Yew -- The mammalian cell cycle / Jane Vera Harper and Gavin Brooks -- Synchronization of cell populations in G1/S and G2/M phases of the cell cycle / Jane Vera Harper -- Mapping origins of DNA replication in eukaryotes / Susan A. Gerbi -- In situ assay for analyzing the chromatin binding of proteins in fission yeast / Stephen E. Kearsey [and others] -- Application of magnetic beads to purify cells transiently transfected with plasmids encoding short hairpin RNAs / Peter D. Adams -- The cell cycle and virus infection / Steven Emmett [and others] -- Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies / Julie Welburn and Jane Endicott -- E2F transcription factors and pRb pocket proteins in cell cycle progression / Ludger Hhauck and Rüdiger von Harsdorf -- Forkhead (FOX) transcription factors and the cell cycle / Katrina A. Bicknell -- Measurement of geminin activity in xenopus egg extracts / Thomas J. McGarry -- CDK-activating kinases / Stéphane Larochelle and Robert P. Fisher -- Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors / Gavin Brooks -- Measurement of wee kinase activity / Paul R. Mueller and Walter F. Leise III -- CDC25 dual-specificity protein phosphatases / Sorab N. Dalal and Melanie Volkening -- Assaying cell cycle checkpoint / Carmela Palermo and Nancy C. Walworth -- Polo-like kinase-1 / Marcel A.T.M. van Vugt and René H. Medema -- The Ipl1/aurora kinase family / Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor -- Purification of the Ndc80 kinetochore subcomplex from xenopus eggs / Mark L. McCleland and P. Todd Stukenberg
Summary Our understanding of the cell cycle and its regulation has significantly increased with recent discoveries of links to DNA repair, growth, cellular metabolism, development, cell death, proliferative disease, cardiovascular diseases, and cancer. In Cell Cycle Control: Mechanisms and Protocols, internationally recognized researchers not only provide overviews of cell cycle regulatory mechanisms, but also set forth their best techniques for studying the eukaryotic cell cycle and its key regulatory molecules in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators-and those more recently defined-in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell Cycle Control: Mechanisms and Protocols offers all cell researchers an indispensable collection of critical reviews and techniques essential to investigate how normal cells divide and how this is altered in disease
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Cell cycle -- Laboratory manuals
Molecular Biology -- methods
Cell Cycle -- physiology
Eukaryotic Cells -- physiology
SCIENCE -- Life Sciences -- Developmental Biology.
Cell cycle
Ciclo celular.
Células eucarióticas.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Humphrey, Tim (Tim Carter)
Brooks, Gavin
ISBN 1588291448