| Description |
1 online resource (x, 405 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 59 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 59
|
| Contents |
General strategies / Shawn Doonan -- Preparation of extracts from animal tissues / Shawn Doonan -- Protein extraction from plant tissues / Peter R. Shewry, Roger J. Fido -- Extraction of recombinant protein from bacteria / D. Margaret Worrall -- Protein extraction from fungi / Paul Bridge -- Subcellular fractionation of animal tissues / Norma M. Ryan -- Subcellular fractionation of plant tissues : isolation of chloroplasts and mitochondria from leaves / Alyson K. Tobin -- The extraction of enzymes from plant tissues rich in phenolic compounds / William S. Pierpoint -- Avoidance of proteolysis in extracts / Robert J. Beynon, Simon Oliver -- Concentration of extracts / Shawn Doonan -- Making and changing buffers / Shawn Doonan -- Purification and concentration by ultrafiltration / Paul Schratter -- Bulk purification by fractional precipitation / Shawn Doonan -- Ion-exchange chromatography / David Sheehan, Richard FitzGerald -- Hydrophobic interaction chromatography / Paul O'Farrell -- Affinity chromatography / Paul Cutler -- Dye-ligand affinity chromatography / D. Margaret Worrall -- Lectin affinity chromatography / Iris West, Owen Goldring -- Immunoaffinity chromatography / George W. Jack, David J. Beer -- Immobilized metal ion affinity chromatography / Tai-Tung Yip, T. William Hutchens -- Chromatography on hydroxyapatite / Shawn Doonan -- Affinity precipitation methods / Jane A. Irwin, Keith F. Tipton -- Isoelectric focusing / Reiner Westermeier -- Chromatofocusing / Timothy J. Mantle, Patricia Noone -- Size-exclusion chromatography / Paul Cutler -- Fast protein liquid chromatography (FPLC) methods / David Sheehan -- Reversed-phase chromatography of proteins / Bill Neville -- Extraction of membrane proteins / Kay Ohlendieck -- Removal of detergent from protein fractions / Kay Ohlendieck -- Purification of membrane proteins / Kay Ohlendieck -- Lyophilization of proteins / Ciarán Ó. Fágáin -- Storage of pure proteins / Ciarán Ó. Fágáin -- Electroelution of proteins from polyacrylamide gels / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels / Michael J. Dunn -- High-performance electrophoresis chromatography / Serge Desnoyers, Sylvie Bourassa, Guy G. Poirier -- Practical column chromatography / Shawn Doonan |
| Summary |
A comprehensive collection of essential, time-tested recipes for successful protein fractionation and purification in any experimental circumstance. The protocols give step-by-step instructions on how to select a source for the protein of interest, how to obtain a usable initial extract, how to purify the protein from that extract using both chemical and molecular methods, and how to dry and store the purified protein. Protein Purification Protocols provides all that is needed to design and carry out a successful purification program. It helps both experienced and novice investigators to clarify and define their purification problems and then provides a comprehensive set of tools for a practical solution |
| Analysis |
eiwitten |
|
proteins |
|
biologie |
|
biology |
|
scheiding |
|
separation |
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fractionering |
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fractionation |
|
zuiveren |
|
purification |
|
analytische methoden |
|
analytical methods |
|
ionenuitwisseling |
|
ion exchange |
|
handleidingen |
|
guide books |
|
Proteins and Enzymes |
|
Biological Techniques |
|
Eiwitten en enzymen |
|
Biologische technieken |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
|
English |
|
Print version record |
|
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Proteins -- Purification -- Laboratory manuals
|
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Proteins -- isolation & purification
|
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Proteins -- Purification.
|
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Eiwitten.
|
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Zuiveringstechnieken.
|
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Extractie (scheikunde)
|
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Affiniteitschromatografie.
|
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Chromatografie.
|
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Wetenschappelijke technieken.
|
| Genre/Form |
Laboratory manuals.
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Doonan, Shawn.
|
| ISBN |
9781592595457 |
|
1592595456 |
|
1280836660 |
|
9781280836664 |
|
9786610836666 |
|
6610836663 |
|
