| Description |
xv, 212 pages : illustrations ; 26 cm |
| Series |
The experimenter series |
|
Experimenter series.
|
| Contents |
Machine derived contents note: Table of Contents -- Acknowledgement -- v -- Foreword -- Vii -- Abbreviations -- Ix -- 1 Introduction -- 1.1 Terminology -- 1.2 Design and development of microarrays . -- 1 2 3 -- 2 Applications -- 2.1 Scientific problems -- 2.2 The pharmaceutical goal. -- 2.3 Demands of bio-technical companies -- 7 7 -- 10 12 -- 3 Technology -- 3.1 Protein microarrays -- 3.1.1 Gene- and protein expression -- 3.1.2 Protein sundries: from molecule to biochip . -- 3.1.3 Protein chips -- 3.2 Nucleic acid microarrays -- 3.2.1 Hybridization specimens -- 3.2.2 Minimal quantities of RNA, what to do? -- 3.2.3 Fabrication of DNA-Microarrays . -- 3.3 Microarray-Detection -- 3.3.1 Light and radiation -- 3.3.2 Luminescent fluorophore T. -- 3.3.3 High Enerhy Laser Light . -- 3.3.4 Microarray Scanner ¿ Mode of Operation. -- 3.3.5 Operating Mode of Microarray Imagers -- 3.3.6 Dynamic range. -- 3.3.7 Pixels and spots -- 3.4 Microarray Marking Systems -- 3.4.1 Tyramide-Signal-Amplification -- 3.4.2 Dendrimers -- 3.4.3 Quantum-Dots -- 3.4.4Chemiluminescence 3.4.5 Radioactivity... -- 3.4.6 Time-Resolve-Fluorescence -- 15 15 15 24 40 54 55 62 70 75 76 78 83 86 90 91 92 94 94 95 96 98 99 99 -- 4 Instrumentation and Software -- 4.1 Microarray Spotter -- 4.1.1 Mechanical Contact-Spotting¿¿. -- 105 106 -- 109 -- XIV Index -- 4.2 -- 4.3 -- 4.4 -- 4.5 -- 4.1.2 Piezo-Dispenser-Technology ¿. -- 4.1.3 Grids and Spots -- Digitizing ¿The Microarray-Scanner -- 4.2.1 The Microarray-Imager. -- 4.2.2 The Microarray-Scanner -- Microarray Software and Documentation -- 4.3.1 Documentation of Microarray Experiments -- 4.3.2 Design of the Experiment -- 4.3.3 Validation of the Results. -- 4.3.4 Microarray Software -- 4.3.5 Comparison of Expression Data -- 4.3.6 Advanced Analysis. -- Additional Laboratory Equipment and Requirements for Microarray Applications -- 4.4.1 Supplies -- 4.4.2 Additonal Helpful Laboratory Equipment -- Clean Room Technology -- 4.5.1 Cleanliness Classes -- 4.5.2 Clean Room Systems -- 112 115 116 -- 117 117 117 118 120 121 123 129 130 -- 136 136 137 137 138 139 -- 5 Execution of the Microarray Experiment -- 5.1 Isolation and Preparation of Nuc1eic Acids -- 5.1.1 Isolation of RNA -- 5.1.2 mRNA-Isolation from Eukaryotes -- 5.1.3 mRNA-Isolation from Bacteria -- 5.1.4 Specimen Fabrication with the Aid of cDNA Synthesis . -- 5.1.5 Hybridization on the DNA Biochip -- 5.1.6 Pre-Amplification with T7-RNA-Polymerase . -- 5.1.7 Fabrication of the Microarray Samples. -- 5.1.8 Quantification ¿ Inspection ¿ Reliability -- 5.1.9 Hybridization of the Samples. -- 5.1.10 Poly-L-lysine-coating -- 5.2 Protein Array Protocols -- 5.2.1 Protein Array Buffers -- 5.2.2 Verification with Antibodies . -- 5.2.3 Protein Kinase Array: Verification with 33p -- 6 High Throughput Screening -- 6.1 Laboratory Automation -- 6.1.1 Laboratory Automation Software -- 6.1.2 Preparation of Samples -- 6.1.3 Analysis and Reporting . -- 6.2 Drug-Target-Screening -- 6.3 Clinical Chemistry and Genetic-Screening -- 7 Database Research and Patents -- 7.1 Database Research -- 143 143 143 144 144 145 146 146 -- 148 149 149 151 151 152 154 -- 155 -- 157 158 160 161 163 164 165 -- 169 -- 169 -- Index. xv -- 7.2 Patents -- 7.2.1 Patent Research -- 7.2.2 Patent Protected Technologies . -- 7.3 Brands and Trade-Marks -- 170 172 179 182 -- 8 Prospects for the Future -- 8.1 Technical Developments -- 8.2 Requirement Profiles -- 8.3 The End of the End -- 185 185 187 189 -- 9 Appendix -- 9.1 Common Buffers. -- 9.1.1 Spotting- and Washing Buffers . -- 9.1.2 Block Solutions -- 9.1.3 Hybridization Solutions.. -- 9.2 Formula for the calculation annealing-temperature of oligonucleotides . -- 9.2.1 Formula for oligonucleotides up to 15 bases -- 9.2.2 Formula for oligonucleotides from 20 to 70 bases -- 9.3 Mo1 Specification and Calculations. -- 9.3.1 Definition of a mol -- 9.3.2 Definition of molar mass und mo1arity . -- 9.3.3 pmol quantities for nucleic acids -- 9.3.4 Definition of the ¿Dalton' unit. -- 9.3.5 pmol quantities for proteins and peptides -- 9.4 Ca1culation of the Microarray-Spot-Surface Area -- 9.5 Ca1culation of theMicroarray-Spot-Density -- 9.5.1 Spot-density based on center-to-center-distance . -- 9.5.2 Spot-density based on the number of abso1ute spots per cm2 -- 9.6 Ca1cu1ation of Fluorophore- or Target-Density -- 191 191 191 191 192 192 192 192 -- 192 -- 192 -- 192 193 194 194 195 -- 195 -- 195 -- 195 -- 196 -- Company register -- 197 -- WEB-Links -- 201 -- Index -- 203 |
| Notes |
Formerly CIP. Uk |
| Bibliography |
Includes bibliographical references (page 201) and index |
| Subject |
DNA microarrays.
|
|
Gene Expression Profiling -- instrumentation.
|
|
Oligonucleotide Array Sequence Analysis -- methods.
|
| Author |
Röder, Thomas.
|
| LC no. |
2005024163 |
| ISBN |
9780120885435 paperback |
|
0120885433 paperback |
|
