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Book Cover
E-book

Title Gene regulatory networks : methods and protocols / edited by Bart Deplancke, Nele Gheldof
Published New York : Springer : Humana, ©2012

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Description 1 online resource (xi, 446 pages) : illustrations
Series Methods in molecular biology, 1940-6029 ; 786
Methods in molecular biology (Clifton, N.J.) ; v. 786. 1064-3745
Contents How do you find transcription factors? Computational approaches to compile and annotate repertoires of regulators for any genome / Juan M. Vaquerizas, Sarah A. Teichmann, and Nicholas M. Luscombe -- Expression pattern analysis of regulatory transcription factors in Caenorhabditis elegans / Huiyun Feng, Hannah L. Craig, and Ian A. Hope -- High-throughput SELEX determination of DNA sequences bound by transcription factors in vitro / Nobuo Ogawa and Mark D. Biggin -- Convenient determination of protein-binding DNA sequences using quadruple 9-mer-based microarray and DsRed-monomer fusion protein / Min-Jeong Kim [and others] -- Analysis of specific protein-DNA interactions by bacterial one-hybrid assay / Marcus B. Noyes -- MITOMI : a microfluidic platform for in vitro characterization of transcription factor-DNA interaction / Sylvie Rockel, Marcel Geertz, and Sebastian J. Maerkl -- Detecting protein-protein interactions with the split-ubiquitin sensor / Alexander Dunkler, Judith Muller, and Nils Johnsson -- Genome-wide dissection of posttranscriptional and posttranslational interactions / Mukesh Bansal and Andrea Califano -- Linking cellular signalling to gene expression using EXT-encoded reporter libraries / Anna Botvinik and Moritz J. Rossner -- Sample preparation for small RNA massive parallel sequencing / Willemijn M. Gommans and Eugene Berezikov -- CAGE (cap analysis of gene expression) : a protocol for the detection of promoter and transcriptional networks / Hazuki Takahashi [and others] -- Detecting DNaseI-hypersensitivity sites with MLPA / Thomas Ohnesorg, Stefanie Eggers, and Stefan J. White -- Detecting long-range chromatin interactions using the chromosome conformation capture sequencing (4C-seq) method / Nele Gheldof [and others] -- Analyzing transcription factor occupancy during embryo development using ChIP-seq / Yad Ghavi-Helm and Eileen E.M. Furlong -- Genome-wide profiling of DNA-binding proteins using barcode-based multiplex solexa sequencing / Sunil Kumar Raghav and Bart Deplancke -- Computational analysis of protein-DNA interactions from ChIP-seq data / Jacques Rougemont and Felix Naef -- Using a yeast inverse one-hybrid system to identify functional binding sites of transcription factors / Jizhou Yan and Shawn M. Burgess -- Using cisTargetX to predict transcriptional targets and networks in Drosophila / Delphine Potier [and others] -- Proteomic methodologies to study transcription factor function / Harry W. Jarrett -- High-throughput gateway-compatible yeast one-hybrid screen to detect protein-DNA interactions / Korneel Hens, Jean-Daniel Feuz, and Bart Deplancke -- BioTapestry : a tool to visualize the dynamic properties of gene regulatory networks / William J.R. Longabaugh -- Implicit methods for qualitative modeling of gene regulatory networks / Abhishek Garg [and others]
Summary Gene regulatory networks play a vital role in organismal development and function by controlling gene expression. With the availability of complete genome sequences, several novel experimental and computational approaches have recently been developed which promise to significantly enhance our ability to comprehensively characterize these regulatory networks by enabling the identification of respectively their genomic or regulatory state components, or the interactions between these two in unprecedented detail. Divided into five convenient sections, Gene Regulatory Networks: Methods and Protocols details how each of these approaches contributes to a more thorough understanding of the composition and function of gene regulatory networks, while providing a comprehensive protocol on how to implement them in the laboratory. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Gene Regulatory Networks: Methods and Protocols aims to provide novices and experienced researchers alike with a comprehensive and timely toolkit to study gene regulatory networks from the point of data generation to processing, visualization, and modeling
Analysis biomedische wetenschappen
biomedicine
humane genetica
human genetics
genexpressie
gene expression
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Genetic regulation -- Laboratory manuals
Gene expression -- Laboratory manuals
Gene regulatory networks.
Gene expression.
Transcription factors.
Gene Regulatory Networks
Gene Expression
Transcription Factors
Transcription factors
Gene regulatory networks
Gene expression
Genetic regulation
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Deplancke, Bart.
Gheldof, Nele.
ISBN 9781617792922
1617792926