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Book Cover
E-book

Title Bacterial artificial chromosomes / edited by Kumaran Narayanan
Edition 2nd ed
Published New York : Humana Press : Springer, ©2015

Copies

Description 1 online resource (xi, 348 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1227
Methods in molecular biology (Clifton, N.J.) ; v. 1227. 1064-3745
Contents From selective full-length genes isolation by TAR cloning in yeast to their expression from HAC vectors in human cells / Natalay Kouprina [and others] -- Recombineering linear BACs / Qingwen Chen and Kumaran Narayanan -- BAC sequencing using pooled methods / Christopher A. Saski [and others] -- Making BAC transgene constructs with lambda-red recombineering system for transgenic animals or cell lines / Scott Holmes [and others] -- Directing enhancer-traps and iTol2 end-sequences to deleted BAC ends with loxP- and lox511-Tn10 transposons / Pradeep K. Chatterjee -- Recombining overlapping BACs into single large BACs / George Kotzamanis and Athanassios Kotsinas -- Generation of knockout alleles by RFLP based BAC targeting of polymorphic embryonic stem cells / Tahsin Stefan Barakat and Joost Gribnau -- Herpesvirus mutagenesis facilitated by infectious bacterial artificial chromosomes (iBACs) / Karl E. Robinson and Timothy J. Mahony -- Conversion of BAC clones into binary BAC (BIBAC) vectors and their delivery into basidiomycete fungal cells using Agrobacterium tumefaciens / Shawkat Ali and Guus Bakkeren -- Infectious delivery of alphaherpesvirus bacterial artificial chromosomes / Kurt Tobler and Cornel Fraefel -- Recombineering-based gene tagging system for arabidopsis / Jose M. Alonso and Anna N. Stepanova -- BAC transgenic zebrafish for transcriptional promoter and enhancer studies / Petra Kraus, Cecilia L. Winata, and Thomas Lufkin -- BACs-on-beads (BoBs) assay for the genetic evaluation of prenatal samples and products of conception / Francesca Romana Grati, François Vialard, and Susan Gross -- New BAC probe set to narrow down chromosomal breakpoints in small and large derivative chromosomes, especially suited for mosaic conditions / Ahmed B. Hamid [and others] -- BAC-probes applied for characterization of fragile sites (FS) / Kristin Mrasek [and others] -- Application of BAC-Probes to Visualize Copy Number Variants (CNVs) / Anja Weise [and others] -- Site-specific Integration of Bacterial Artificial Chromosomes into Human Cells / Bradley McColl, Sara Howden, and Jim Vadolas -- Generation of BAC Reporter Cell Lines for Drug Discovery / Betty R. Kao, Bradley McColl, and Jim Vadolas
Summary Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls. Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies
Analysis bacteria
celbiologie
cellular biology
moleculaire genetica
molecular genetics
chromosomen
chromosomes
Genetics (General)
Bacteria
Genetica (algemeen)
Bibliography Includes bibliographical references and index
Subject Bacterial artificial chromosomes -- Laboratory manuals
Bacterial artificial chromosomes.
Gene mapping.
Molecular cloning.
Gene libraries.
Chromosomes, Artificial, Bacterial
Chromosome Mapping
Cloning, Molecular
Genomic Library
Medical genetics.
Cellular biology (cytology)
Medical -- Genetics.
Science -- Life Sciences -- Biology -- Molecular Biology.
Molecular cloning
Gene mapping
Gene libraries
Bacterial artificial chromosomes
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Narayanan, Kumaran
ISBN 9781493916528
1493916521
1493916513
9781493916511