| Description |
1 online resource (x, 318 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 68 |
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Methods in molecular biology (Clifton, N.J.) ; v. 68
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| Contents |
Gene mapping goes from FISH to surfing the net / John Valdes, Danilo A. Tagle -- Linkage analysis of genetic disorders / Eugene W. Taylor, Jianfeng Xu, Ethylin Wang Jabs, Deborah A. Meyers -- Gene ordering and localization by linkage analysis / R.E. March -- Gene mapping using somatic cell hybrids / David P. Kelsell, Nigel K. Spurr -- Gene mapping by FISH / Rafael Espinosa, Michelle M. Beau -- Probe ordering and distancing by FISH / Marian Kroef, Hans Dauwerse, Jim Landegent -- Physical mapping by pulsed-field gel electrophoresis / John Maule -- Construction and use of YAC contigs in disease regions / Stephen Horrigan, Carol Westbrook -- Construction and use of cosmid contigs / Nicholas Fairweather -- Use of dinucleotide polymorphism analyses in physical mapping / Jeff Fairman, Lalitha Nagarajan -- Mapping expressed sequence tags (ESTs) by multiplexing PCR reactions from hybrid cell panels and detecting fluorescently labeled products / A. Scott Durkin, Donna R. Maglott, William C. Nierman -- Gene isolation by exon trapping / David B. Krizman -- Isolation of coding sequences from genomic regions using direct selection / Richard G. Mastro, Michael Lovett -- Isolation of cDNAs using the YAC hybridization screen method / Carrie Fidler, Jacqueline Boultwood -- Detection and isolation of differentially expressed genes by differential display / Weimin Zhu, Peng Liang -- Chemical crosslinking subtraction (CCLS) / Ian N. Hampson, Lynne Hampson -- Gene mapping and isolation : access to databases / Martin J. Bishop -- Software for genetic linkage analysis : an update / Stephen P. Bryant -- Exon detection by similarity searches / Jean-Michel Claverie |
| Summary |
An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Gene mapping -- Laboratory manuals
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Gene mapping.
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Chromosome Mapping
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Gene mapping.
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Genanalyse.
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Genetische manipulatie.
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals.
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Boultwood, Jacqueline.
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| ISBN |
9781592595549 |
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1592595545 |
|
128083675X |
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9781280836756 |
|
