| Description |
1 online resource (x, 260 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; v. 262 |
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Methods in molecular biology (Clifton, N.J.) ; v. 262
|
| Contents |
Determination of mitotic recombination rates by fluctuation analysis in Saccharomyces cerevisiae / Rachelle Miller Spell and Sue Jinks-Robertson -- Determination of intrachromosomal recombination rates in cultured mammalian cells / Jason A. Smith and Alan S. Waldman -- Intrachromosomal homologous recombination in Arabidopsis thaliana / Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta -- Analysis of recombinational repair of DNA double-strand breaks in mammalian cells with I-SceI nuclease / Jac A. Nickoloff and Mark A. Brenneman -- Transformation of monomorphic and pleomorphic Trypanosoma brucei / Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry -- Forward genetic screens for meiotic and mitotic recombination-defective mutants in mice / Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima -- Detecting carcinogens with the yeast DEL assay / Richard J. Brennan and Robert H. Schiestl |
|
In vivo DNA deletion assay to detect environmental and genetic predisposition to cancer / Ramune Reliene, Alexander J.R. Bishop, Jiri Aubrecht, and Robert H. Schiestl -- Gene targeting at the chromosomal immunoglobulin locus: a model system for the study of mammalian homologous recombination mechanisms / Mark D. Baker -- DNA fragment transplacement in Saccharomyces cerevisiae: some genetic considerations / Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis -- Targeted gene modification using triplex-forming oligonucleotides / Jean Y. Kuan and Peter M. Glazer -- Using nucleases to stimulate homologous recombination / Dana Carroll -- Enhancement of in vivo targeted nucleotide exchange by nonspecific carrier DNA / Katie K. Maguire and Eric B. Kmiec -- The use of the chromatin immunoprecipitation to investigate protein-DNA interactions during recombination / Tamara Goldfarb and Eric Alani -- Holliday junction branch migration and resolution assays / Angelos Constantinou and Stephen C. West |
| Summary |
Genetic recombination plays an essential role in such vitally important biological processes as DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. In Genetic Recombination: Reviews and Protocols, prominent international investigators actively engaged in recombination research describe in great detail their best techniques for studying recombination. The methods range from approaches and model systems to be used in a variety of eukaryotic organisms and in a mammalian parasite, to biochemical analyses useful for furthering the understanding of recombination mechanisms. Additional techniques use recombination as a reporter of genomic instability in lower and higher eukaryotes and as a tool for producing targeted genetic modification. Each readily reproducible method includes step-by-step instructions, a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Genetic Recombination: Reviews and Protocols offers both novice and experienced investigators an illuminating synthesis of what we currently know about recombination, as well as a wide-ranging collection of powerful tools for elucidating its mechanisms and regulation throughout biology today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Genetic recombination.
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Gene expression.
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Gene targeting.
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Genetic engineering.
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Recombination, Genetic
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Gene Expression
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Gene Targeting
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Genetic Engineering
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SCIENCE -- Life Sciences -- Molecular Biology.
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Genetic engineering
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Gene targeting
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Gene expression
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Genetic recombination
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Waldman, Alan S.
|
| ISBN |
1588292363 |
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9781588292360 |
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1592597610 |
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9781592597611 |
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1280358289 |
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9781280358289 |
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9786610358281 |
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6610358281 |
|
