| Description |
1 online resource (xix, 375 pages) : illustrations |
| Contents |
Gene silencing by RNA interference and the role of small interfering RNAs -- Basics of siRNA design and chemical synthesis -- Oligonucleotide scanning arrays in the design of small interfering RNAs -- siRNA production by in vitro transcription -- Production of siRNAs with the application of deoxyribozymes -- Production of siRNA in vitro by enzymatic digestion of double-stranded RNA -- Plasmid-mediated intracellular expression of siRNAs -- Lentiviral vector-mediated delivery of si/shRNA -- Exogenous siRNA delivery: protocols for optimizing delivery to cells -- RNAi in drosophila cell cultures -- RNAi in caenorhabditis elegans -- Delivery of RNAi reagents in C. elegans by microinjection -- RNAi in drosophila embryos -- RNAi in xenopus laevis oocytes -- RNAi in xenopus embryos -- An RNAi-based genomic library for forward genetics in the African trypanosome -- RNAi in the malaria parasite plasmodium -- Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum using RNA interference approaches -- RNAi in transgenic animal models -- RNA interference in mouse models -- Virus-induced RNA silencing in plants -- RNA silencing in plants: biolistic delivery of RNAi reagents -- Agro-infiltration: a versatile tool for RNAi studies in plants |
| Summary |
Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms.; From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Gene silencing -- Laboratory manuals
|
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RNA -- Laboratory manuals
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RNA interference.
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Gene Silencing
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RNA Interference
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SCIENCE -- Biotechnology.
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RNA interference
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Gene silencing
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RNA
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| Genre/Form |
Electronic books
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Sohail, Muhammad
|
| ISBN |
020348925X |
|
9780203489253 |
|
1280614862 |
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9781280614866 |
|
