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E-book

Uniform Title Marquerurs moléculaires en génétique et biotechnologies végt́ales. English
Title Molecular markers in plant genetics and biotechnology / editor, Dominique de Vienne
Published Enfield, NH : Science Publishers, ©2003

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Description 1 online resource (xii, 235 pages) : illustrations
Contents What is a 'good' genetic marker? -- 1. Principal sources of molecular markers / D. de Vienne, S. Santoni and M. Falque -- Criteria of classification -- Codominant markers detected individually -- Sequence polymorphism -- Differences at restriction enzyme sites: the RFLP technique -- particular case of RFLP: CAPS -- Differences of conformation: SSCP -- Differences of stability: D/TGGE -- SNP genotyping techniques -- Polymorphism of number of sequence repeats: SSR -- Patterns of multiple dominant markers: genetic fingerprinting -- Sequence polymorphism -- Differences in hybridization sites of an arbitrary primer, or techniques of MAAP, RAPD, AP-PCR and DAF -- Differences of restriction sites and hybridization sites of specific primers: AFLP -- Polymorphism of number of tandem repeats -- ISSR techniques -- Minisatellites -- Gene markers: cDNA and proteins -- What markers are suitable for what purpose? -- 2. Construction of genetic linkage maps / D. de Vienne -- concept of genetic distance -- Haldane distance -- Kosambi distance and other distances -- Estimation of recombination rates and linkage tests -- Comparing the most commonly used populations -- Populations giving access to the haploid phase -- Doubled haploids -- Megagametophyte of gymnosperms -- Backcross populations -- F[subscript 2] populations -- Recombinant inbred lines -- Populations derived from non-fixed parents -- General case -- double pseudo-testcross -- Comparing the various types of populations -- Perenniality -- Estimation of dominance -- Accuracy -- Fundamentals of genetic maps -- Saturation of maps -- Relativity of map lengths -- Relations between genetic and physical distances -- Analysis of genome organization -- Comparative mapping -- 3. Mapping of major genes / D. de Vienne -- Approaches to mapping of major genes -- Near-isogenic lines -- Bulked segregant analysis (BSA) -- Use of markers for cloning major genes -- Principle of positional cloning -- High-resolution mapping -- Looking for the target gene in genomic clones -- 4. Mapping and characterization quantitative trait loci / D. de Vienne and M. Causse -- Principle of QTL mapping -- Detection of QTL considering markers individually -- Finding QTL by analysis of variance in an F[subscript 2] population -- Measures of QTL effect -- Limits of QTL detection on individual markers -- Detection of QTLs from two or more markers -- Methods of interval mapping -- Multiple marker methods -- Factors influencing QTL detection -- Detection power and within-class variation -- Population size and marker density -- Choice of statistical risks -- Deviation from normality -- Advantages and disadvantages of populations currently used for QTL detection -- Comparison of F[subscript 2], backcross, double haploids, and recombinant inbred lines -- Progeny derived from heterozygous parents -- Populations in which the genetic background is partly fixed -- Selective genotyping -- Genetic and molecular bases of quantitative trait variation -- Genetic data on QTLs -- QTLs are (almost) always detected -- Dominance effects -- Transgression -- Epistasis -- Genetic analysis of correlation between traits -- Genetic dissection of traits -- Effect of the environment -- Comparative QTL mapping -- Characterizing QTLs -- QTL cloning -- Candidate genes -- Validation of candidate genes -- 5. Molecular markers in population genetics / A. Kremer and S. Mariette -- Specific contributions of molecular markers in comparison to Traditional markers -- Multialelism -- Increase in the number of loci -- Access to polymorphism of cytoplasmic DNA -- Ordering of alleles -- Analysis of molecular diversity -- Measurement of nucleotide divergence from sequences -- Measurement of nucleotide divergence using marker techniques -- Polymorphism within a population -- Expression of polymorphism within a population -- Indexes taking into account the number of variants -- Indexes taking into account the frequency of variants -- Measurement of genetic diversity with different types of markers -- At the allelic level -- At the nucleotide level -- Differentiation between populations -- General expression of differentiation -- Measurement of differentiation with different marker systems -- At the allele level -- At the nucleotide level -- Gene flow -- Indirect measures of gene flow -- Comparative rates of seed and pollen migration -- Origin of colonizing genotypes -- Direct measures of gene flow -- 6. Application of markers in selection / A. Charcosset and A. Gallais -- Contribution of diversity studies to selection -- Principal methods of data analysis -- Relationships between molecular divergence, phenotypic divergence and coancestry -- Application of classification methods: heterotic groups and genetic resource management -- Relationship between molecular divergence and heterosis -- Use of markers to protect new varieties -- Perspectives -- Marker-assisted selection -- Genotype building -- Marker-assisted backcrossing for a monogenic trait -- Marker-assisted backcrossing for a polygenetic trait -- Marker-assisted pedigree selection -- Marker-assisted recurrent selection -- Recurrent selection on markers alone -- Recurrent selection on additive value predicted by markers -- Combined selection based on phenotype and markers -- Comparison with construction of genotypes -- Choice between use of markers and increase in the number of replications
Bibliography Includes bibliographical references (pages 185-229) and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Print version record
Subject Plant genome mapping.
Genetic polymorphisms.
Genes, Plant
Molecular Biology
Plants -- genetics
Polymorphism, Genetic
Genetic polymorphisms
Plant genome mapping
Genetik
Biotechnologie
Markierungsgen
Pflanzen
Biotecnologia vegetal.
Marcadors bioquímics.
Form Electronic book
Author Vienne, D. de (Dominique)