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Title Recombinant gene expression protocols / edited by Rocky S. Tuan
Published Totowa, N.J. : Humana Press, [1997]


Location Call no. Vol. Availability
 W'PONDS  572.877 Tua/Rge  AVAILABLE
Description xx, 521 pages : illustrations (some color) ; 24 cm
Series Methods in molecular biology ; v. 62
Methods in molecular biology (Clifton, N.J.) ; v. 62
Contents 1. Introduction -- Recombinanat gene expression protocols overview of experimental strategies for the expression of recombinant genes -- 2. Expression in E. coli -- Designing expression plasmid vectors in E. coli -- Design of bacterial hosts for Iac-based expressed vectors -- Bacteriophage gamma-based expression vectors -- Strategies for gene fusions -- Application of the E. coli trp promoter -- Expression of proteins in E. coli utilizing a dual promoter-based vector: pLACT7 -- Expression and secretion of proteins in E. coli -- Synthetic two-cistron expression system -- Expression of cDNA-encoded proteins by cell-free transcription and translation -- 3. Expression in yeast -- Yeast plasids -- Inducible expression cassettes in yeast: GAL4 -- Inducible expression cassettes in yeast: ADH2 -- Constitutive expression vectors: PGK -- 4. Viral expression systems -- Design and construction of recombinant vaccinia viruses -- A simplified method of screening for isolation of recombinant vaccinia virus -- Design of retroviral expression vectors -- Use of defective herpes-derived plasmid vectors -- 5. Expression in nonmammalian higher eukaryotes -- Use of baculovirus expression vectors -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos -- Analysis of heterologous gene expression in Xenopus blastomeres -- 6. Expression in mammalian cells -- Overview of vector design for mammalian gene expression -- Experimental strategies in efficient transfection of mammalian cells: calcium phosphate and DAAE-dextran -- Experimental strategies in efficient transfection of mammalian cells: electroporation -- Highly effective delivery of foreign DNA to atherent cells via polybrene/DMSO-assisted gene transfer -- Selection of transfected cells: magnetic affinity cell sorting -- Selection of transfected cells and coamplification of transfected genes -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells -- 7. Expression in transgenic organisms -- 8. Expression in Plants
Analysis Genetic recombination Laboratory manuals
Gene Expression
Genetic Engineering
Laboratory Manuals
Recombination, Genetic
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to (BMP) via Humana Press
Methods in molecular biology (Clifton, N.J.) no:62 1064-3745
Subject Genetic recombination -- Laboratory manuals.
Recombination, Genetic.
Gene Expression.
Genetic Engineering.
Genre/Form Laboratory Manual.
Laboratory manuals.
Author Tuan, Rocky S.
Humana Press.
LC no. 96049600
ISBN 0896033333 (alk. paper)