Title MHC protocols / edited by Stephen H. Powis, Robert W. Vaughan

Published Totowa, N.J. : Humana Press, ©2003

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Description 1 online resource (xii, 340 pages) : illustrations Series Methods in molecular biology ; v. 210 Methods in molecular biology (Clifton, N.J.) ; v. 210. Contents I. Databases -- HLA Informatics : Accessing HLA Sequences from Sequence Databases / James Robinson, Steven G.E. Marsh -- Accessing HLA Sequencing Data Through the 6ace Database / Roger Horton, Stephan Beck -- II. Polymorphism in Classical and Nonclassical HLA Genes -- HLA Typing by Restriction Fragment Length Polymorphism Analysis / Robert W. Vaughan -- PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles / Robert W. Vaughan -- PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR / Derek Middleton, F. Williams -- HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes / Lori L. Steiner [and others] -- PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles / Mike Bunce -- HLA Typing With Reference Strand-Mediated Conformation Analysis / J. Rafael Arguello [and others] -- Sequencing Protocols for Detection of HLA Class I Polymorphism / Paul P.J. Dunn, Steven T. Cox, Ann-Margaret Little -- HLA-E and HLA-G Typing / Jorge Martinez-Laso, Eduardo Gomez-Casado, Antonio Arnaiz-Villena -- Typing Alleles of HLA-DM / Helene Teisserenc -- III. Polymorphism in Non-HLA MHC Genes -- Typing Alleles of TAP1 and TAP2 / Stephen H. Powis -- Determining Alleles of the C2 Gene by Southern Blotting / Zeng-Bian Zhu, John E. Volanakis -- Complement C4 Protein and DNA Typing Methods / Peter M. Schneider, Gottfried Mauff -- Typing of Tumor Necrosis Factor Alleles / Anthony Gerard Wilson -- Molecular Typing of the MHC Class I Chain-Related Gene Locus / R.W.M. Collins, Henry A.F. Stephens, Robert W. Vaughan -- IV. Microsatellites -- HLA Microsatellite Analysis / Mary Carrington Summary Many of the genes found in the human major histocompatibility complex (MHC:HLA region) are subject to extreme polymorphism. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. Many MHC genes also have significant disease association and play a pivotal role in the control of immune response. In MHC Protocols, leading experts detail readily reproducible methods for identifying these polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP2, C2, C4, and TNF-a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles. Each method is written by an investigator who has used the technique extensively and includes troubleshooting tips and notes on pitfalls to avoid. Comprehensive and highly practical, MHC Protocols offers both experienced and novice investigators not only a state-of-the-art overview of this dramatically evolving area of immunology, but also a reliable guide to the identification of MHC gene polymorphisms Bibliography Includes bibliographical references and index Notes English In Springer Protocols Subject Major histocompatibility complex -- Genetic aspects -- Laboratory manuals Immunogenetics -- Laboratory manuals Genetic polymorphisms -- Laboratory manuals Major histocompatibility complex. HLA histocompatibility antigens. Genetic polymorphisms. Major Histocompatibility Complex HLA Antigens Polymorphism, Genetic MEDICAL -- Immunology. Major histocompatibility complex HLA histocompatibility antigens Genetic polymorphisms Immunogenetics Major histocompatibility complex -- Genetic aspects Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Powis, Stephen H Vaughan, Robert W ISBN 0896035484 9780896035485 1592592910 9781592592913 1280830751 9781280830754 9786610830756 6610830754

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