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Title Gene transfer and expression protocols / edited by E.J. Murray
Published Clifton, N.J. : Humana Press, ©1991

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Description 1 online resource (xiii, 439 pages) : illustrations
Series Methods in molecular biology ; v. 7
Methods in molecular biology (Clifton, N.J.) ; v. 7
Contents Preparation of recombinant plasmid DNA for DNA-mediated gene transfer / Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Calcium phosphate mediated gene transfer into established cell lines / Hiroto Okayama, Claudia Chen -- Transfection of the Chloramphenicol-acetyltransferase gene into eukaryotic cells using diethyl-aminoethyl (DEAE)-dextran / Richard A. Lake, Michael J. Owen -- Polybrene/DMSO-assisted gene transfer / Remy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Electroporation technique of DNA transfection / Sarah C. Spencer -- Irradiation and fusion gene transfer / Peter N. Goodfellow -- Direct use of [lambda] phage particles for DNA transfection / Masahiro Ishiura -- Cationic liposome-mediated transfection with Lipofectin reagent / Philip L. Felgner -- Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection / Michael A. Tainsky -- Chromosome-mediated gene transfer / Janet M. Shipley, Denise Sheer -- Manipulation of adenovirus vectors / Frank L. Graham, Ludvik Prevec -- Manipulation of vaccinia virus vectors / Michael Mackett -- Manipulation of baculovirus vectors / Murk J. Bailey, Robert D. Possee -- Manipulation of SV40 vectors / Marie-Louise Hammurskjöld -- Choice and manipulation of retroviral vectors / Jay P. Morgenstern, Hartmut Land -- Use of tissue-plasminogen activator as a reporter gene / Nicholas C. Wrighton -- Use of Escherichia coli (E. coli) lacZ ([beta]-galactosidase) as a reporter gene / Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg -- Application of the firefly luciferase reporter gene / Vincent Giguère -- Use of vectors to confer resistance to antibiotics G418 and hygromycin in stably transfected cell lines / Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell -- Selection of cells defective in pyrimidine (TK⁻) and purine (APRT⁻ and HPRT⁻ salvage : development of host strains appropriate for transfection / Mark Meuth, Janet Harwood -- Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay / Maggie E. Walmsley, Roger K. Patient -- The RNase protection assay / Mitchell H. Finer -- Primer extension analysis of mRNA isolated from transfected cell lines / Mark W. Leonard, Roger K. Patient -- Northern blot analysis of gene expression / Robb Krumlauf -- Analysis of transcriptional initiation in isolated nuclei / David Stott -- Immunoperoxidase staining of gene products in cultured cells using monoclonal antibodies / Roger Morris -- The use of flow cytometry to detect transfected gene products / Raymond Bujdoso, David Sargan, Keith Ballingall, Andrew Sanderson -- Determination of foreign gene copy number in stably transfected cell lines by Southern transfer analysis / Ronald M. Fourney, Rémy Aubin, Kevin D. Dietrich, Makolm C. Paterson -- Evaluation of extrachromosomal gene copy number of transiently transfected cell lines / Angus C. Wilson, Roger K. Patient -- Use of Dpn I restriction enzyme to assess newly replicated gene copies in amplifiable vector systems / Alison C. Brewer, Roger K. Patient -- Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines / Andreas Zimmer, Peter Gruss -- Induction of erythroid-specific expression in murine erythroleukemia (MEL) cell lines / Michael Antoniou
Summary The purpose of this handbook is to detail most of the molecular biology techniques used to introduce an isolated gene into a cell line, using a variety of transfection techniques
Analysis gentransfer
gene transfer
genexpressie
gene expression
transfectie
transfection
genetische vectoren
genetic vectors
dna
genen
genes
biologische technieken
biological techniques
Molecular Biology (General)
Moleculaire biologie (algemeen)
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
In Springer Protocols
Subject Genetic transformation -- Technique
Transfection -- Technique
Gene expression -- Technique
Gene expression.
Gene Expression
Molecular Biology -- methods
Gene expression
Gene expression -- Technique
Genetic transformation -- Technique
Transfection -- Technique
Regulering.
DNA.
Wetenschappelijke technieken.
Transfert génétique -- Technique.
Transfection génétique.
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Murray, E. J.
ISBN 9781592594948
1592594948