Title Carbohydrate biotechnology protocols / edited by Christopher Bucke

Published Totowa, N.J. : Humana Press, ©1999

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Description 1 online resource (xii, 337 pages) : illustrations Series Methods in biotechnology, 1940-607X ; 10 Methods in biotechnology ; 10. 1940-6061 Contents Introduction to carbohydrate biotechnology -- Production and isolation of xanthan gum -- Alginate from Azotobacter vinelandii -- Production of schizophyllan -- Enzymatic synthesis of cellulose -- Modification of alginate using mannuronan C-5 epimerases -- Viscosity control of guar polysaccharide solutions by treatment with galactose oxidase and catalase enzymes -- The production of cyclodextrins using CGTase from bacillus macerans -- Production of microbial glycolipids -- Partial enzymatic hydrolysis of starch to maltodextrins on the laboratory scale -- The production of [alpha](1-2)-terminated glucooligosaccharides -- Enzymatic production of fructooligosaccharides from sucrose -- Enzymatic production of inulooligosaccharides from inulin -- One-pot enzymatic synthesis of sialyl T-epitope -- Hydrolysis of hemicelluloses using combinations of xylanases and feruloyl esterases -- Enzymatic depolymerization of chitins and chitosans -- Synthesis of homo- and hetero-oligosaccharides from underivatized sugars using glycosidases -- Use of fluorophore-assisted carbohydrate electrophoresis (FACE®) in the elucidation of n-linked oligosaccharide structures -- Application of sucrose synthase in the synthesis of nucleotide sugars and saccharides -- Production of isomaltulose using immobilized bacterial cells -- The production of mannitol by fermentation -- The production of 3-keto-derivatives of disaccharides -- Enzymatic synthesis of [alpha]-butylglucoside in a biphasic butanol-water system using the [alpha]-transglucosidase from Aspergillus niger -- Enzymatic glycosylation of aglycones of pharmacological significance -- Enzymatic synthesis of glycosides in aqueous-organic two-phase systems and supersaturated substrate solutions -- Use of [beta]-glucosidase in the development of flavor in wines and fruit juices Summary In Carbohydrate Biotechnology Protocols, Christopher Bucke has brought together a compilation of modern hands-on methods for the effective use of microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Protocols offers synthetic chemists, biochemists, fermentation biotechnologists, and applied enzymologists cutting-edge techniques-many of them hitherto unavailable in print-that are cleaner and often less costly than available chemical alternatives. Timely and readily reproducible, these state-of-the-art protocols allow the user to produce and Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Carbohydrates -- Biotechnology -- Laboratory manuals Carbohydrates -- biosynthesis Carbohydrates -- chemical synthesis Biotechnology -- methods SCIENCE -- Biotechnology. Carbohydrates -- Biotechnology Biotechnologie Kohlenhydrate Glucides -- Biotechnologie -- Manuels de laboratoire. Polysaccharides -- Biotechnologie -- Manuels de laboratoire. Oligosaccharides -- Biotechnologie -- Manuels de laboratoire. Carbohydrates. Biotechnologie -- Manuels de laboratoire. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Bucke, C ISBN 0585241031 9780585241036 0896035638 9780896035638 9781592592616 1592592619

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