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Book Cover

Title Ionotropic glutamate receptor technologies / edited by Gabriela K. Popescu
Published New York, NY : Humana Press, [2015]


Description 1 online resource (xii, 304 pages) : illustrations (some color)
Series Neuromethods, 0893-2336 ; 106
Neuromethods ; 106. 0893-2336
Contents Assaying AMPA Receptor Oligomerization -- A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells -- Counting NMDA Receptors at the Cell Surface -- Electron Microscopy Analysis of AMPA receptors in Dendritic Spines -- Electrophysiological Tagging of Ionotropic Receptors -- Functional Detection of Novel Triheteromeric NMDA Receptors -- Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses -- NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains -- Computing Conformational Free Energies of iGluR Ligand-Binding Domains -- LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors -- Assaying the Energetics of NMDA Receptor Pore Opening -- Constructing a Rapid Solution Exchange System -- Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics -- Analysis of Whole-Cell NMDA Receptor Responses -- Expression, Purification and Crystallization of Full Length Ionotropic Glutamate Receptors -- Timing AMPA Receptor Activation with Laser-pulse Photolysis -- Current Recording and Kinetic Analyses for Single AMPA Receptors -- Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings
Summary This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology. Chapters in the book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time
Notes Includes index
Online resource; title from PDF title page (SpringerLink, viewed October 7, 2015)
Subject Glutamic acid -- Receptors -- Laboratory manuals
Ion channels -- Laboratory manuals
Neurotransmitters -- Laboratory manuals
Ion channels.
Neurotransmitter receptors.
Receptors, Glutamate -- physiology
Ion Channels
Receptors, Neurotransmitter
Medical -- Physiology.
Medical -- Neuroscience.
Neurotransmitter receptors
Glutamic acid -- Receptors
Ion channels
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Popescu, Gabriela K., editor
ISBN 9781493928125