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Book Cover
Author Leach, Mike

Title Practical Flow Cytometry in Haematology Diagnosis
Published Chicester : Wiley, 2013


Description 1 online resource (258 pages)
Contents Practical Flow Cytometry in Haematology Diagnosis; Copyright; Contents; Preface; Acknowledgements; Chapter 1 Introduction; References; Chapter 2 Principles of Flow Cytometry; Introduction; Sample preparation; Direct staining method for surface immunophenotyping of peripheral blood or bone marrow (lyse -- no wash method); Surface immunoglobulin staining; Intracellular Staining; The flow cytometer; The fluidic system; The optical system; Light scatter; Fluorescence; Fluorescence intensity; Spectral overlap; The electronic system; Threshold; Data display; Histogram; Dot plot
Instrument set-up and quality controlAcknowledgements; References; Chapter 3 Limitations; Introduction; Clinical context issues; Sampling issues; Blood; Bone marrow aspirates; Effusions; Cerebrospinal fluid; Regenerating bone marrows; Technical issues; Operator training; Antibody and fluorochrome selection; Background fluorescence; Interpretation issues; Morphological correlation; Clinical context; Combined report; Conclusion; References; Chapter 4 Normal Blood and Bone Marrow Populations; Normal stem and precursor cell populations; Haematopoietic progenitor cells; Myeloid maturation
Erythroid maturationMegakaryocytic maturation; Lymphoid maturation; Haematogones; References; Chapter 5 Acute Leukaemia; Introduction; Acute leukaemia: definition and classification; Clinical presentation and sampling for flow cytometry; Identification of neoplastic precursor cells; The blast population; Blast populations are heterogeneous; Correlating the immunophenotype with genetic analysis; Blast cell characteristics; Patterns of precursor antigen expression in acute leukaemia; Enumeration of blast cells; Lineage assignment of acute leukaemia; Lineage assignment in acute myeloid leukaemia
Lineage assignment in acute lymphoblastic leukaemiaAcute leukaemia of ambiguous lineage; Acute myeloid leukaemia; Aberrant and asynchronous antigen expression in acute myeloid leukaemia; Antigenic patterns of particular significance in acute myeloid leukaemia; CD34 -negative HLA-DR-negative acute myeloid leukaemia; Identifying particular acute myeloid leukaemia subtypes by flow; Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma); B-cell acute lymphoblastic leukaemia/lymphoma; Aberrant antigen expression in B-acute lymphoblastic leukaemia
Identifying B-acute lymphoblastic leukaemia subtypes by flowT-cell acute lymphoblastic leukaemia/lymphoma; Aberrant antigen expression in T-acute lymphoblastic leukaemia; Examination of cerebro-spinal fluid and serous effusions; Central Nervous System Disease in Acute Leukaemia; Detection of acute leukaemia cells in serous effusions; Identification of genetic abnormalities by flow; Conclusion; References; Chapter 6 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma; Normal peripheral blood lymphoid populations; Identification of clonal lymphoid populations
Summary This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples
Notes Identification of clonal B-cell disorders
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Blood -- Diseases -- Diagnosis.
Flow cytometry -- Methodology
Hematology -- Methodology
Blood -- Tumors -- Diagnosis
Hematologic Diseases -- diagnosis
Hematologic Neoplasms -- diagnosis
Flow Cytometry -- methods
Hematology -- methods
Blood -- Diseases -- Diagnosis
Flow cytometry -- Methodology
Form Electronic book
Author Drummond, Mark
Doig, Allyson
ISBN 9781118487990