| Description |
1 online resource (xvi, 551 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; v. 1124 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1124. 1064-3745
|
| Contents |
The role of neutrophils in the immune system : an overview / Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn -- Isolation of human neutrophils from venous blood / William M. Nausef -- Neutrophil isolation from nonhuman species / Daniel W. Siemsen ... [and 7 others] -- Collection of in vivo transmigrated neutrophils from human skin / Karin Christenson ... [and 6 others] -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard -- Rho family and Rap GTPase activation assays / Richard T. Jennings and Ulla G. Knaus -- Measurement of phospholipid metabolism in intact neutrophils / Susan Sergeant and Linda C. McPhail -- Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils / Maurice B. Hallett, Maha Al-Jumaa, and Sharon Dewitt -- Analysis of electrophysiological properties and responses of neutrophils / Deri Morgan and Thomas E. DeCoursey -- Assessment of neutrophil apoptosis / David A. Dorward ... [and 3 others] -- Microinjection methods for neutrophils / Iraj Laffafian ... [and 3 others] -- Generation of functionally mature neutrophils from induced pluripotent stem cells / Colin L. Sweeney ... [and 6 others] -- Neutrophil migration through extracellular matrix / Richard T. Jennings and Ulla G. Knaus -- Spinning disk confocal imaging of neutrophil migration in zebrafish / Pui-Ying Lam ... [and 4 others] -- Detection of bidirectional signaling during integrin activation and neutrophil adhesion / Stuart M. Altman, Neha Dixit, and Scott I. Simon -- Immunofluorescence and confocal microscopy of neutrophils / Lee-Ann H. Allen -- Expressin of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils / Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein -- Quantitative assessment of neutrophil phagocytosis using flow cytometry / Pontus Nordenfelt -- Analysis of neutrophil bactericidal activity / Heather A. Parker ... [and 4 others] -- Induction and quantification of neutrophil extracellular traps / Alejandro Sanchez Gonzalez ... [and 3 others] -- Measurement of respiratory burst products, released or retained, during activation of professional phagocytes / Johan Bylund ... [and 4 others] -- Cell-free NADPH oxidase activation assays : "In vitro veritas" / Edgar Pick -- Assessment of priming of the human neutrophil respiratory burst / Margarita Hurtado-Nedelec ... [and 4 others] -- Affinity pruification and reconstitution of human phagocyte flavocytochrome b for detection of conformational dynamics in the membrane / Marcia Riesselman and Algirdas J. Jesaitis -- Evaluation of p47phox phosphorylation in human neutrophils using phospho-specicfic antibodies / Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna -- Genome-scale transcript analyses with human neutrophils / Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils / Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni -- High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis / Flavia S. Lakschevitz and Michael Glogauer -- Detection of intact transciption factors in human neutrophils / Patrick P. McDonald and Richard D. Ye -- Disorders of neutrophil function : an overview / Mary C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / Houda Zghal Elloumi and Steven M. Holland -- Diagnostic assays for myeloperoxidase and myeloperoxidase deficiency / William M. Nauseef |
| Summary |
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells, and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity, and extracellular trap formation; and protocols for investigating neutrophil adhesion, chemotaxis, and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils |
| Analysis |
geneeskunde |
|
medicine |
|
volksgezondheid |
|
public health |
|
microorganisms |
|
genomica |
|
genomics |
|
infectieziekten |
|
infectious diseases |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Online resource; title from PDF title page (SpringerLink, viewed Feb. 17, 2014) |
| Subject |
Neutrophils -- Laboratory manuals
|
|
Phagocytes.
|
|
Granulocytes.
|
|
Leucocytes.
|
|
Immune system.
|
|
Cells.
|
|
Anatomy.
|
|
Blood cells.
|
|
Blood.
|
|
Neutrophils.
|
|
Phagocytes
|
|
Granulocytes
|
|
Leukocytes
|
|
Immune System
|
|
Cells
|
|
Myeloid Cells
|
|
Anatomy
|
|
Blood Cells
|
|
Hemic and Immune Systems
|
|
Blood
|
|
Neutrophils
|
|
Immune System Phenomena
|
|
Animal Structures
|
|
anatomy.
|
|
blood (animal material)
|
|
Phagocytes
|
|
Leucocytes
|
|
Immune system
|
|
Granulocytes
|
|
Cells
|
|
Blood cells
|
|
Blood
|
|
Anatomy
|
|
Neutrophils
|
|
Neutrophiler Granulozyt
|
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Neutrophiles.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Quinn, Mark Thomas, 1958-
|
| LC no. |
2014930433 |
| ISBN |
9781627038454 |
|
1627038450 |
|
1627038442 |
|
9781627038447 |
|
