Description |
1 online resource (xiv, 377 pages) : illustrations |
Series |
Methods in Molecular Biology ; 1051 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1051. 1064-3745
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Contents |
New opportunities for immobilization of enzymes / Jose M. Guisan -- Immobilization of enzymes : a literature survey / Beatriz Brena, Paula González-Pombo, and Francisco Batista-Viera -- Glutaraldehyde-mediated protein immobilization / Fernando López-Gallego, Jose M. Guisán, and Lorena Betancor -- Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports / Cesar Mateo, Valeria Grazu, and Jose M. Guisan -- Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups / Fernando López-Gallego [and others] -- Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports / Cesar Mateo [and others] -- Reversible covalent immobilization of enzymes via disulfide bonds / Karen Ovsejevi, Carmen Manta, and Francisco Batista-Viera -- Immobilization of Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles for biocatalysis in low-water media / Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta -- Immobilization of enzymes by bioaffinity layering / Veena Singh, Meryam Sardar, and Munishwar Nath Gupta -- Immobilization of enzymes on magnetic beads through affinity interactions / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Tips for the functionalization of nanoparticles with antibodies / Ester Polo [and others] -- Design and characterization of functional nanoparticles for enhanced bio-performance / Pablo del Pino, Scott G. Mitchell, and Beatriz Pelaz -- Immobilization of enzymes on ethynyl-modified electrodes via click chemistry / Akhtar Hayat [and others] -- Modification of carbon nanotube electrodes with 1-pyrenebutanoic acid, succinimidyl ester for enhanced bioelectrocatalysis / Guinevere Strack [and others] -- Enzyme immobilization by entrapment within a gel network / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Practical protocols for lipase immobilization via sol-gel techniques / Manfred T. Reetz -- Improving lipase activity by immobilization and post-immobilization strategies / Jose M. Palomo [and others] -- High activity preparations of lipases and proteases for catalysis in low water containing organic solvents and ionic liquids / Ipsita Roy [and others] -- Biomedical applications of immobilized enzymes : an update / Marta Pastor, Amaia Esquisabel, and José Luis Pedraz -- Immobilization of whole cells by chemical vapor deposition of silica / Susan R. Sizemore [and others] -- Encapsulation of cells in alginate gels / Pello Sánchez [and others] -- Microalgal immobilization methods / Ignacio Moreno-Garrido -- Therapeutic applications of encapsulated cells / Argia Acarregui [and others] -- Whole cell entrapment techniques / Jorge A. Trelles and Cintia W. Rivero |
Summary |
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells |
Analysis |
cellen |
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cells |
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celbiologie |
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cellular biology |
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enzymologie |
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enzymology |
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immobilisatie |
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immobilization |
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immobilized cells |
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enzymen |
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enzymes |
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protocollen |
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protocols |
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Proteins and Enzymes |
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Eiwitten en enzymen |
Bibliography |
Includes bibliographical references and index |
Notes |
English |
Subject |
Immobilized enzymes -- Biotechnology
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Enzymes.
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Technology.
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Life sciences.
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Cells.
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Physical sciences.
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Proteins.
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Anatomy.
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Methodology.
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Immobilized enzymes.
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Immobilized cells.
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Publication Formats
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Enzymes
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Immobilized Proteins
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Technology
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Biological Science Disciplines
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Investigative Techniques
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Cells
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Technology, Industry, and Agriculture
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Enzymes and Coenzymes
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Natural Science Disciplines
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Proteins
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Anatomy
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Publication Characteristics
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Disciplines and Occupations
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Technology, Industry, Agriculture
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Amino Acids, Peptides, and Proteins
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Chemicals and Drugs
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Methods
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Biotechnology
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Laboratory Manuals
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Enzymes, Immobilized
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Cells, Immobilized
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enzyme.
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biological sciences.
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physical sciences.
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protein.
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anatomy.
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methodology.
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bioengineering.
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Technology
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Proteins
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Physical sciences
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Methodology
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Life sciences
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Immobilized enzymes
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Immobilized cells
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Enzymes
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Cells
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Anatomy
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Immobilized enzymes -- Biotechnology
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Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Guisan, Jose M.
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ISBN |
9781627035507 |
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1627035508 |
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1627035494 |
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9781627035491 |
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