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E-book

Title Immobilization of enzymes and cells / edited by Jose M. Guisan
Edition 3rd ed
Published New York : Humana Press : Springer, ©2013

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Description 1 online resource (xiv, 377 pages) : illustrations
Series Methods in Molecular Biology ; 1051
Methods in molecular biology (Clifton, N.J.) ; v. 1051. 1064-3745
Contents New opportunities for immobilization of enzymes / Jose M. Guisan -- Immobilization of enzymes : a literature survey / Beatriz Brena, Paula González-Pombo, and Francisco Batista-Viera -- Glutaraldehyde-mediated protein immobilization / Fernando López-Gallego, Jose M. Guisán, and Lorena Betancor -- Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports / Cesar Mateo, Valeria Grazu, and Jose M. Guisan -- Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups / Fernando López-Gallego [and others] -- Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports / Cesar Mateo [and others] -- Reversible covalent immobilization of enzymes via disulfide bonds / Karen Ovsejevi, Carmen Manta, and Francisco Batista-Viera -- Immobilization of Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles for biocatalysis in low-water media / Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta -- Immobilization of enzymes by bioaffinity layering / Veena Singh, Meryam Sardar, and Munishwar Nath Gupta -- Immobilization of enzymes on magnetic beads through affinity interactions / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Tips for the functionalization of nanoparticles with antibodies / Ester Polo [and others] -- Design and characterization of functional nanoparticles for enhanced bio-performance / Pablo del Pino, Scott G. Mitchell, and Beatriz Pelaz -- Immobilization of enzymes on ethynyl-modified electrodes via click chemistry / Akhtar Hayat [and others] -- Modification of carbon nanotube electrodes with 1-pyrenebutanoic acid, succinimidyl ester for enhanced bioelectrocatalysis / Guinevere Strack [and others] -- Enzyme immobilization by entrapment within a gel network / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Practical protocols for lipase immobilization via sol-gel techniques / Manfred T. Reetz -- Improving lipase activity by immobilization and post-immobilization strategies / Jose M. Palomo [and others] -- High activity preparations of lipases and proteases for catalysis in low water containing organic solvents and ionic liquids / Ipsita Roy [and others] -- Biomedical applications of immobilized enzymes : an update / Marta Pastor, Amaia Esquisabel, and José Luis Pedraz -- Immobilization of whole cells by chemical vapor deposition of silica / Susan R. Sizemore [and others] -- Encapsulation of cells in alginate gels / Pello Sánchez [and others] -- Microalgal immobilization methods / Ignacio Moreno-Garrido -- Therapeutic applications of encapsulated cells / Argia Acarregui [and others] -- Whole cell entrapment techniques / Jorge A. Trelles and Cintia W. Rivero
Summary For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells
Analysis cellen
cells
celbiologie
cellular biology
enzymologie
enzymology
immobilisatie
immobilization
immobilized cells
enzymen
enzymes
protocollen
protocols
Proteins and Enzymes
Eiwitten en enzymen
Bibliography Includes bibliographical references and index
Notes English
Subject Immobilized enzymes -- Biotechnology
Enzymes.
Technology.
Life sciences.
Cells.
Physical sciences.
Proteins.
Anatomy.
Methodology.
Immobilized enzymes.
Immobilized cells.
Publication Formats
Enzymes
Immobilized Proteins
Technology
Biological Science Disciplines
Investigative Techniques
Cells
Technology, Industry, and Agriculture
Enzymes and Coenzymes
Natural Science Disciplines
Proteins
Anatomy
Publication Characteristics
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Disciplines and Occupations
Technology, Industry, Agriculture
Amino Acids, Peptides, and Proteins
Chemicals and Drugs
Methods
Biotechnology
Laboratory Manuals
Enzymes, Immobilized
Cells, Immobilized
enzyme.
biological sciences.
physical sciences.
protein.
anatomy.
methodology.
bioengineering.
Technology
Proteins
Physical sciences
Methodology
Life sciences
Immobilized enzymes
Immobilized cells
Enzymes
Cells
Anatomy
Immobilized enzymes -- Biotechnology
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Guisan, Jose M.
ISBN 9781627035507
1627035508
1627035494
9781627035491