Title Recombinant antibodies for cancer therapy : methods and protocols / edited by Martin Welschof and Jürgen Krauss

Published Totowa, N.J. : Humana Press, ©2003

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Description 1 online resource (xiv, 481 pages) : illustrations Series Methods in molecular biology ; v. 207 Methods in molecular biology (Clifton, N.J.) ; v. 207 Contents Generation of antibody molecules through antibody engineering / S.M. Kipriyanov -- Application of recombinant antibodies in cancer patients / J. Krauss, M. Arndt and M. Pfreundschuh -- DNA immunization as a means to generate antibodies to proteins / P.S. Chowdhury -- Chimerization of a monoclonal antibody for treating hodgkin's lymphoma / J. Krauss [and others] -- Humanization of monoclonal antibodies by CDR grafting / S. O'Brien and T. Jones -- Generation and screening of a modular human scFv expression library from multiple donors / M. Welschof [and others] -- Construction of semisynthetic antibody libraries / M. Braunagel -- Single-domain VH antibody fragments from a phage display library / L. Binyamin, D. Plaksin and Y. Reiter -- Isolation of human fab fragments against ovarian carcinoma using guided selection / M. Figini [and others] -- Human recombinant fab antibodies with t-cell receptor-like specificities generated from phage display libraries / J. Engberg [and others] -- Engineering hot spots for affinity enhancement of antibodies / P.S. Chowdhury -- Simultaneous humanization and affinity optimization of monoclonal antibodies / H. Wu -- Tailoring kinetics of antibodies using focused combinatorial libraries / H. Wu and L.L. An -- Engineering scFvs for improved stability / P.S. Chowdhury and G. Vasmatzis -- Recombinant single-chain and disulfide-stabilized Fv immunotoxins for cancer therapy / R. Niv, D. Segal and Y. Reiter -- Generation of recombinant immunotoxins for specific targeting of tumor-related peptides presented by MHC molecules / C.J. Cohen [and others] -- Construction and characterization of RNase-based targeted therapeutics / D.L. Newton [and others] -- Bispecific diabodies for cancer therapy / M. Arndt and J. Krauss -- Generation and characterization of bispecific tandem diabodies for tumor therapy / S.M. Kipriyanov -- Generation of recombinant multimeric antibody fragments for tumor diagnosis and therapy / B.E. Power, A.A. Kortt and P.J. Hudson -- Construction and characterization of minibodies for imaging and therapy of colorectal carcinomas / P.J. Yazaki and A.M. Wu -- Generation, expression, and monitoring of recombinant immune receptors for use in cellular immunotherapy / A. Hombach, C. Heuser and H. Abken -- Tailoring natural effector functions. Antibody engineering beyond humanization / O.H. Brekke and J.E. Thommesen -- Single-chain Fv-based affinity purification of the cellular stress protein GP96 for vaccine development / C. Kleist [and others] -- Recombinant adenoviruses for in vivo expression of antibody fragments / R.E. Kontermann, T. Korn and V. Jerome -- Production of antibody fragments in a bioreactor / H. Hellebust -- Large scale production of recombinant antibodies by utilizing cellulose-binding domains / I. Benhar and Y. Berdichevsky -- Production of tumor-specific antibodies in tobacco / C. Vaquero-Martin and R. Fischer Summary Many antibodies recently developed as therapeutic agents have been tailored by the powerful new recombinant antibody technology, and already their clinical data show highly encouraging results. In Recombinant Antibodies for Cancer Therapy: Methods and Protocols, Martin Welschof and Jürgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of those novel anticancer therapeutics. Written by seasoned investigators who have successfully verified the particular method described, these readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. The main focus is on providing detailed protocols that describe these procedures step-by-step, accompanied by a troubleshooting guide discussing possible problems and offering many tips for their potential solutions. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering. Up-to-date and highly practical, Recombinant Antibodies for Cancer Therapy: Methods and Protocols surveys the current status of engineered antibodies and describes in rich detail many readily reproducible methods of generating the novel and promising antibody-based reagents of today's cancer therapy Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Cancer -- Immunotherapy. Recombinant antibodies -- Laboratory manuals Antibodies -- therapeutic use Neoplasms -- therapy Antibodies -- immunology Antigens, Neoplasm -- immunology Antineoplastic Protocols Immunoglobulin Fragments -- therapeutic use Immunotherapy -- methods Neoplasms -- immunology Recombinant Proteins -- therapeutic use MEDICAL -- Oncology. HEALTH & FITNESS -- Diseases -- Cancer. Cancer -- Immunotherapy Recombinant antibodies Kanker. Recombinant antibodies. Wetenschappelijke technieken. Genre/Form Laboratory manuals Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Welschof, Martin. Krauss, Jürgen. ISBN 9781592593347 0896039188 9780896039186 1592593348 1280842172 9781280842177 9786610842179 6610842175

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