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Book Cover
Author Amit, M. (Michal)

Title Atlas of human pluripotent stem cells : derivation and culturing / Michal Amit, Joseph Itskovitz-Eldor ; with contributions by Ilana Laevsky and Atara Novak
Published New York : Humana Press, ©2012


Description 1 online resource (xiv, 142 pages) : illustrations (some color)
Series Stem cell biology and regenerative medicine
Stem cell biology and regenerative medicine.
Contents Methods for the Derivation of Human Embryonic Stem Cell Lines -- Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders -- Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Conditions -- Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures -- Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bodies -- Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation -- Immunostaining -- Karyotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines -- Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes
Summary Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. ¡ The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cell research
Analysis Life sciences
Stem cells
biologische technieken
biological techniques
cellular biology
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Subject Stem cells -- Atlases
Pluripotent Stem Cells
Cell Culture Techniques
Induced Pluripotent Stem Cells
HEALTH & FITNESS -- Diseases -- General.
MEDICAL -- Clinical Medicine.
MEDICAL -- Diseases.
MEDICAL -- Evidence-Based Medicine.
MEDICAL -- Internal Medicine.
Stem cells.
Genre/Form atlases.
Scientific atlases.
Form Electronic book
Author Itskovitz-Eldor, Joseph
Laevsky, Ilana
Novak, Atara
ISBN 9781617795480