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Book Cover
Author Lee, Yee-Ki.

Title Calcium handling in hiPSC-derived cardiomyocytes / Lee Yee-Ki, Siu Chung-Wah
Published New York, NY : Springer, ©2012


Description 1 online resource (xii, 52 pages) : illustrations (some color)
Series SpringerBriefs in stem cells, 2192-8118
SpringerBriefs in stem cells.
Contents Calcium Handling in hiPSC-Derived Cardiomyocytes -- Myocardial Infarction and Heart Failure -- Embryonic Stem Cells/Pluripotent Stem Cells -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Cardiomyocytes as a Source for Cardiac Regeneration -- Spontaneous Cardiac Differentiation -- Mesoderm Enrichment by Wnt-Signaling Growth Factors -- Endoderm-Like Cell (END)-2 Co-culture Method -- Calcium Homeostasis in Cardiomyocytes -- Sarcoplasmic Reticulum (SR) Governs Maturity of Cardiomyocytes -- SR Junctional Proteins Play a Role in Calcium Flux Between Cytosol and SR -- Spatial and Temporal Ca2+ Wavefront Dictated by T-Tubule Structure -- IP3-Mediated Calcium Release Contributes to Whole-Cell Calcium Transients -- Calcium Handling Properties of hES-Derived Cardiomyocytes -- Calcium Handling Properties of hiPSC-Derived Cardiomyocytes -- iPSC-Derived Cardiomyocyte as a Potential Platform for Disease Modeling of the Impaired Calcium Handling-Related Syndrome -- Disease Modeling of Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) -- Mitochondrial Dysfunction-Related Cardiomyopathy-Impaired SR Ca2+ Reuptake -- Measurement of Cytosolic [Ca2+] by Fluorescence Confocal Microscopy -- Materials -- Calibration of Fluo-3 -- Isolation of hiPSC- and hESC-Derived Cardiomyocytes -- Recording of Cytosolic [Ca2+] -- RT-qPCR of Calcium Handling Proteins
Summary Annotation Calcium is crucial in governing contractile activities of myofilaments in cardiomyocytes, any defeats in calcium homeostasis of the cells would adversely affect heart pumping action. The characterization of calcium handling properties in human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMCs) is of significant interest and pertinent to the stem cell and cardiac regenerative field because of their potential patient-specific therapeutic use
Analysis Life sciences
Stem cells
Cell Biology
cellular biology
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Subject Heart cells.
Stem cells.
Calcium -- Physiological effect.
Calcium -- physiology
Induced Pluripotent Stem Cells
Myocytes, Cardiac
Stem Cells
MEDICAL -- Surgery -- Transplant.
Calcium -- Physiological effect.
Heart cells.
Stem cells.
Form Electronic book
Author Siu, Chung-Wah.
ISBN 9781461440932