Title Myeloid leukemia : methods and protocols / edited by Harry Iland, Mark Hertzberg, Paula Marlton

Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (xi, 306 pages) : illustrations Series Methods in molecular medicine ; 125 Methods in molecular medicine ; 125. Contents Isolation of RNA and DNA from leukocytes and cDNA synthesis / Joop H. Jansen and Bert A. van der Reijden -- Cytogenetic and fish techniques in myeloid malignancies / Lynda J. Campbell -- Overview of real-time RT-PCR strategies for quantification of gene rearrangements in the myeloid malignancies / Christophe Picard, Monique Silvy, and Jean Gabert -- Diagnosis and monitoring of chronic myeloid leukemia by qualtitative and quantitative RT-PCR / Susan Branford and Tim Hughes -- Detection of BCR-ABL mutations and resistance to imatinib mesylate / Susan Branford and Tim Hughes -- Deletion of the derivative chromosome 9 in chronic myeloid leukemia / Lynda J. Campbell -- Diagnosis and monitoring of PML-RARA-positive acute promyelocytic leukemia by qualitative RT-PCR / Vincenzo Rossi, Laura Levati, and Andrea Biondi -- Diagnosis and monitoring of PML-RARA-positive acute promyelocytic leukemia by quantitative RT-PCR / Elisa Mokany [and others] Diagnosis and monitoring of AML1MTG8 (ETO)-positive acute myeloid leukemia by qualitative and quantitative RT-PCR / Khalid Tobal and John A. Liu Yin -- Diagnosis and monitoring of CBFB-MYH11-positive acute myeloid leukemia by qualitative and quantitative RT-PCR / Bert A. van der Reijden and Joop H. Jansen -- Detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia / Jan Cools, Elizabeth H. Stover, and D. Gary Gilliland -- FLT3 mutations in acute myeloid leukemia / Hitoshi Kiyoi and Tomoki Naoe -- WT-1 overexpression in acute myeloid leukemia and myelodysplastic syndromes / Daniela Cilloni, Enrico Gottardi, and Giuseppe Saglio -- Classification of AML by DNA-oligonucleotide microarrays / Alexander Kohlmann [and others] -- Classification of AML using a monoclonal antibody microarray / Richard I. Christopherson [and others] Methods for the detection of the JAK2 V617F mutation in human myeloproliferative disorders / Peter J. Campbell [and others] Overexpression of PRV-1 gene in polycythemia rubra vera and essential thrombocythemia / Maurizio Martini, Luciana Teofili, and Luigi M. Larocca -- Chimerism analysis following nonmyeloablative stem cell transplantation / Thomas Lion and Franz Watzinger Summary The highly significant role that acquired genetic abnormalities play in the genesis, diagnosis, and management of hematological malignancies has become increasingly clear. Such abnormalities can serve as useful markers for initial diagnosis, accurate subclassification, and the evaluation of minimal residual disease, as well as providing critical targets for novel therapies. In Myeloid Leukemia: Methods and Protocols, a panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. The methods range from those that are of immediate clinical relevance to the investigation and management of patients with myeloid malignancies, to those that relate to recently identified genetic abnormalities of potential clinical significance. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Clinically relevant and highly practical, Myeloid Leukemia: Methods and Protocols offers cytogeneticists, hematologists, and oncologists cutting-edge laboratory techniques that can be rapidly implemented for the investigation and management of patients with myeloid malignancies Bibliography Includes bibliographical references and index Notes English Subject Acute myeloid leukemia. Chronic myeloid leukemia. Acute diseases. Leukemia, Myeloid -- genetics Acute Disease Leukemia, Myeloid -- diagnosis Molecular Diagnostic Techniques Leukemia, Myeloid, Acute Leukemia, Myelogenous, Chronic, BCR-ABL Positive MEDICAL -- Oncology. HEALTH & FITNESS -- Diseases -- Cancer. Acute diseases Acute myeloid leukemia Chronic myeloid leukemia Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Iland, Harry Hertzberg, Mark Marlton, Paula ISBN 1588294854 9781588294852 1597450170 9781597450171 128035853X 9781280358531 9786610358533 6610358532

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