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Book Cover
E-book

Title Leucocytes : methods and protocols / edited by Robert B. Ashman
Published Totowa, N.J. : Humana ; London : Springer [distributor], 2012

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Description 1 online resource (xi, 296 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 844
Methods in molecular biology (Clifton, N.J.) ; v. 844. 1064-3745
Contents ENU-based phenotype-driven screening -- Detection and quantification of cytokines and other biomarkers -- Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cells -- Flow cytometry measurement of bone marrow perfusion in the mouse and sorting of progenitors and stems cells according to position relative to blood flow in vivo -- Analyzing cell death events in cultured leukocytes -- Towards a four-dimensional view of neutrophils -- Isolation of human and mouse neutrophils ex vivo and in vitro -- Measurement of oxidative burst in neutrophils -- Measurement of neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates -- The macrophage -- Generation and characterization of MacGreen mice, the cfs1r-EGFP transgenic mice -- Generation of mouse bone marrow-derived macrophages -- Isolation and differentiation of monocytes-macrophages from human blood -- In vitro measurement of phagocytosis and killing of Cryptococcus neoformans by macrophages -- Measuring the inflammasome -- Arginine and macrophage activation -- Immunodetection of granzyme B tissue distribution and cellular localisation -- Detection of human and mouse granzyme B activity in cell extracts -- T cell transfer model of colitis: A great tool to assess the contribution of T cells in chronic intestinal inflammation -- Measurement of nitrite in urine by gas chromatography-mass spectrometry
Summary The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Leucocytes:Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes
Analysis leukocyten
leukocytes
celbiologie
cellular biology
immuniteitsreactie
immune response
methodologie
methodology
protocollen
protocols
Molecular Biology (General)
Moleculaire biologie (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Leucocytes -- Laboratory manuals
Immunity.
Immune system.
Blood cells.
Cells.
Blood.
Anatomy.
Leucocytes.
Cellular immunity.
Natural immunity.
Immunity
Adaptive Immunity
Immune System
Publication Formats
Blood Cells
Cells
Hemic and Immune Systems
Publication Characteristics
Immune System Phenomena
Blood
Anatomy
Phenomena and Processes
Laboratory Manuals
Leukocytes
Immunity, Cellular
Immunity, Innate
Animal Structures
blood (animal material)
anatomy.
Natural immunity
Immunity
Immune system
Cellular immunity
Cells
Blood cells
Blood
Anatomy
Leucocytes
Biology.
Human Anatomy & Physiology.
Health & Biological Sciences.
Microbiology & Immunology.
Physiology.
Cytology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ashman, Robert B
ISBN 9781617795275
1617795275