Title Hydrocarbon and lipid microbiology protocols : single-cell and single-molecule methods / Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Published Berlin : Springer, 2016

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Description 1 online resource (x, 204 pages) : illustrations (some color) Series Springer Protocols handbooks, 1949-2448 Springer protocols (Series) 1949-2448 Contents Introduction: Single cell biotechnology for uncultured microorganisms -- Single-cell cultivation in microfluidic devices -- Single-cell bacterial bioreporter assays to measure hydrocarbons -- Whole genome amplification of single bacterial cells -- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria -- Localisation of proteins within intact bacterial cells using fluorescent protein fusions -- Raman-FISH -- Single-cell metabolomics -- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division -- Single cell biotechnology for uncultured microorganisms -- Single cell and systems biology tools for biofuel production -- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) -- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity -- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence Summary Annotation This volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy Bibliography Includes bibliographical references Notes Online resource; title from PDF title page (SpringerLink, viewed April 19, 2016) Subject Lipids -- Laboratory manuals Lipids -- Biotechnology -- Laboratory manuals Hydrocarbons -- Laboratory manuals Bacteria -- Physiology. Metabolomics Hydrocarbons -- metabolism Lipid Metabolism -- physiology Bacterial Physiological Phenomena Single-Cell Analysis Bacteria -- Physiology Hydrocarbons Lipids Lipids -- Biotechnology Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author McGenity, Terry J., editor Timmis, Kenneth N., editor Nogales, Balbina, editor ISBN 9783662491317 3662491311 366249129X 9783662491294

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