| Description |
xvii, 498 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; 314 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 314
|
| Contents |
Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / Małgorzata Z. Zdzienicka -- Complementation assays adapted for DNA repair/deficient keratinocytes / Mathilde Fréchet ... [et al.] -- Cytogenetic challenge assays for assessment of DNA repair capacities / William Au and Salama A. Salama -- Evaluating the delayed effects of cellular exposure to ionizing radiation / Shruti Nagar, James J. Corcoran, and William F. Morgan -- Inhibition of DNA synthesis by ionizing radiation: a marker for an S-phase checkpoint / Nicolaas G.J. Jaspers and Małgorzata Z. Zdzienicka -- Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis, Ya Wang, and Hong Yan Wang -- |
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Cytometric assessment of histone H2AX phosphorylation, a reporter of DNA damage / Xuan Huang and Zbigniew Darzynkiewicz -- Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication) / Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi -- In vitro rejoining of double-strand breaks in genomic DNA / George Iliakis and Nge Cheong -- Detection of DNA double-strand breaks and chromosomal translocations using ligation-mediated and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan -- Plasmid-based assays for DNA end joining in vitro / George Iliakis ... [et al.] -- Use of gene targeting to study recombination in mammalian DNA repair mutants / Rodney Nairn and Gerald Adair -- |
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Gene-specific and mitochondrial repair of oxidative DNA damage / R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr -- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells / Janine H. Santos ... [et al.] -- Measuring the formation and repair of DNA damage by ligation-mediated PCR / Gerd P. Pfeifer -- Immunochemical detection of UV-induced DNA damage and repair / Marcus S. Cooke and Alistair Robson -- A dot-blot immunoassay for measuring repair of UV photoproducts / Shirley McCready -- Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / David L. Mitchell -- DNA damage quantitation by alkaline gel electrophoresis / Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland -- |
|
The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair / Günter Speit and Andreas Hartmann -- Fast micromethod DNA single-strand break assay / Heinz C. Schröder ... [et al.] -- 32P-postlabeling DNA-damage assays: PAGE, TLC, and HPLC / Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki -- Electrophoretic mobility shift assays to study protein binding to damaged DNA / Vaughn Smider, Byung Joon Hwang, and Gilbert Chu -- Construction of MMR plasmid substrates and analysis of MMR error correction and excision / Huixian Wang and John B. Hays -- Analysis of enzymes that act at abasic sites in DNA / Walter A. Deutsch and Vijay Hegde -- Base excision repair in mammalian cells / Yoshihiro Matsumoto -- In vitro base excision repair assay using mammalian cell extracts / Guido Frosina ... [et al.] -- |
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Biochemical assays for the characterization of DNA helicases / Robert M. Brosh Jr. and Sudha Sharma -- Repair synthesis assay for nucleotide excision repair activity using fractionated cell extracts and UV-damaged plasmid DNA / Maureen Biggerstaff and Richard D. Wood -- Assaying for the dual incisions of nucleotide excision repair using DNA with a lesion at a specific site / Mahmud K.K. Shivji ... [et al.] -- Analysis of proliferating cell nuclear antigen (PCNA) associated with DNA excision repair sites in mammalian cells / A. Ivana Scovassi and Ennio Prosperi -- Analysis of DNA repair and chromatin assembly in vitro using immobilized damaged DNA substrates / Jill A. Mello, Jonathan G. Moggs, and Geneviève Almouzni |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
| Subject |
DNA damage -- Laboratory manuals.
|
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Mammals -- Genetics -- Laboratory manuals.
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DNA repair -- Laboratory manuals.
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DNA Repair.
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DNA Damage.
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Mammals -- genetics.
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| Genre/Form |
Laboratory Manual.
|
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Laboratory manuals.
|
| Author |
Henderson, Daryl S.
|
|
Humana Press.
|
| LC no. |
2005006250 |
| ISBN |
1588295133 alkaline paper |
|
