Title Developmental biology of the sea urchin and other marine invertebrates : methods and protocols / edited by David J. Carroll, Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL, USA ; Stephen A. Stricker, Department of Biology, University of New Mexico, Albuquerque, NM, USA

Published New York : Humana Press, [2014] ©2014

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Description 1 online resource (xiv, 347 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 1128 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 1128. 1064-3745 Springer protocols (Series) Contents Culturing larvae of marine invertebrates / Richard R. Strathmann -- Obtaining gametes and embryos of ascidians / Charles C. Lambert -- Artificial seawater culture of the gastropod Crepidula fornicate for studies of larval settlement and metamorphosis / Anthony Pires -- Trichoplax adhaerens, an enigmatic basal metazoan with potential / Andreas Heyland, Roger Croll, Sophie Goodall, Jeff Kranyak, and Russell Wyeth -- Rearing gymnolaemate bryozoan larvae for cellular and molecular analyses / Michael H. Temkin -- An invertebrate embryologist's guide to routine processing of confocal images / George von Dassow -- TEM analyses of chaetognath reproductive organs / George L. Shinn -- SEM analysis of marine invertebrate gametes / John Buckland-Nicks -- Imaging neural development in embryonic and larval sea urchins / Oliver Krupke, Shunsuke Yaguchi, Junko Yaguchi, and Robert D. Burke -- Preparation and use of sea urchin egg homogenates / Anthony J. Morgan and Antony Galione -- Microinjection and 4D fluorescence imaging in the eggs and embryos of the ascidian Phallusia mammillata / Alex McDougall, Karen Wing-man Lee, and Remi Dumollard -- Isolating specific embryonic cells of the sea urchin by FACS / Celina Juliano, S. Zachary Swartz, and Gary Wessel -- Biotinylation of oocyte cell surface proteins of the starfish Patiria miniata / Samantha Cihal and David J. Carroll -- Perturbations to the hedgehog pathway in sea urchin embryos / Jacob F. Warner and David R. McClay -- Regulation of DNA synthesis at the first cell cycle in the sea urchin in vivo / Jolanta Kisielewska and Michael Whitaker -- Immunoblotting analyses of changes in protein phosphorylations during oocyte maturation in marine nemertean worms / Jose R. Escalona and Stephen A. Stricker -- Multicolor labeling in developmental gene regulatory network analysis / Aditya J. Sethi, Robert C. Angerer, and Lynne M. Angerer -- A 96-well plate format for detection of marine zooplankton with the sandwich hybridization assay / Julio B.J. Harvey -- Isolation and assessment of signaling proteins from synchronized cultures during egg activation and through the egg-to-embryo transition in sea urchins / Michelle M. Roux-Osovitz and Kathy R. Foltz -- Tools for sea urchin genomic analysis / R. Andrew Cameron -- Antibody inhibition of protein activity in starfish oocytes / Eiichi Okumura, Masatoshi Hara, and Takeo Kishimoto -- Immunophotoaffinity labeling of the binding proteins for 1-methyladenine, an oocyte maturation-inducing hormone of starfish / Tetsuo Kida, Shinjiro Matsuda, Atsushi Kuyama, and Tetsuo Toraya Summary In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates Analysis echinoidea marine invertebrates ontogenie ontogeny levenswetenschappen life sciences celbiologie cellular biology diermodellen animal models Invertebrata Ontogeny, Embryology Ontogenie, embryologie Bibliography Includes bibliographical references and index Notes English Print version record Subject Sea urchins -- Development -- Laboratory manuals Marine invertebrates -- Development -- Laboratory manuals Sea urchins -- Development -- Research -- Methodology Marine invertebrates -- Development -- Research -- Methodology Seas. Echinodermata. Seawater. Sea urchins. Oceans and Seas Echinodermata Animals Organism Forms Organisms Seawater Eukaryota Environment Ecological and Environmental Phenomena Biological Phenomena Phenomena and Processes Invertebrates Aquatic Organisms Sea Urchins seas. Seawater Seas Sea urchins Echinodermata Marine invertebrates -- Development Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Carroll, David J., editor Stricker, Stephen A., editor LC no. 2014931085 ISBN 9781627039741 1627039740

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