| Description |
338 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; 309 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 309
|
| Contents |
Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of drosophila RNA silencing complexes by native gel electrophoresis / John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in C. elegans / Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei / Christine E. Clayton ... [et al.] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing / M. Florian Mette ... [et al.] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sebastiona Wulchli -- Silencing gene expression with dicer-generated siRNA pools / Jason W. Myers and James E. Ferrell, Jr. -- |
|
Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector-based RNAi technology / Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo / Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni ... [et al.] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and Jo Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs / Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elsa Maniataki, Maria Dels Angels de Planell Saguer, and Zissimos Mourelatos -- |
|
Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou ... [et al.] -- High throughput analysis of microRNA gene expression using sensitive cDNA probes / Mouldy Sioud and Øystein Røsok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu ... [et al.] |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Small interfering RNA -- Laboratory manuals.
|
|
Gene silencing -- Laboratory manuals.
|
|
RNA interference.
|
|
Genetic regulation -- Genetics.
|
|
MicroRNA -- Physiology.
|
|
Small interfering RNA -- Physiology.
|
|
RNA Interference.
|
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Gene Expression Regulation -- genetics.
|
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MicroRNAs -- physiology.
|
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RNA, Small Interfering -- physiology.
|
| Genre/Form |
Laboratory manuals.
|
| Author |
Carmichael, Gordon
|
| LC no. |
2004023107 |
| ISBN |
1588294366 alkaline paper |
|
