| Description |
1 online resource (xxii, 344 pages) : illustrations (some color) |
| Contents |
Foreword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization |
| Summary |
Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references |
| Analysis |
Microbiology Laboratory techniques |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Bacteriophages -- Laboratory manuals
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Microbial biotechnology -- Laboratory manuals
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Viral proteins -- Laboratory manuals
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Affinity chromatography -- Laboratory manuals
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Peptides -- Biotechnology -- Laboratory manuals
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Proteins -- Biotechnology -- Laboratory manuals
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Bacteriophages.
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Affinity chromatography.
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Bacteriophages
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Chromatography, Affinity
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Peptides
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Proteins
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protein.
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SCIENCE -- Life Sciences -- Evolution.
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Affinity chromatography
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Bacteriophages
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Microbial biotechnology
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Peptides -- Biotechnology
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Proteins -- Biotechnology
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Viral proteins
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Genexpressie.
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Combinatorische chemie.
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Peptiden.
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Eiwitten.
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Antistoffen.
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Bactériophages.
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Microorganismes -- Biotechnologie.
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Protéines virales.
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Chromatographie d'affinité.
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Technologie des protéines.
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Peptides -- Biotechnologie.
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Protéines.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuals (Handbooks)
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Kay, Brian K.
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Winter, Jill.
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McCafferty, John, Dr.
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| ISBN |
9780124023802 |
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0124023800 |
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9780080538709 |
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0080538703 |
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