| Description |
1 online resource (xi, 256 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1940-6029 ; 1151 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1151. 1064-3745
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| Contents |
Recent progress in engineering human-associated microbiomes / Stephanie J. Yaung, George M. Church, and Harris H. Wang -- Constructing synthetic microbial communities to explore the ecology and evolution of symbiosis / Adam James Waite and Wenying Shou -- Combining engineering and evolution to create novel metabolic mutualisms between species / Lon Chubiz, Sarah Douglas, and William Harcombe -- Design, construction, and characterization methodologies for synthetic microbial consortia / Hans C. Bernstein and Ross P. Carlson -- Observation method for autonomous signaling-mediated synthetic diversification in Escherichia coli / Ryoji Sekine, Shotaro Ayukawa, and Daisuke Kiga -- Integration-free reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) without viral vectors, recombinant DNA, and genetic modification / Boon Chin Heng and Martin Fussenegger -- Transformation of Bacillus subtilis / Xiao-Zhou Zhang, Chun You, and Yi-Heng Percival Zhang -- Culturing anaerobes to use as a model system for studying the evolution of syntrophic mutualism / Sujung Lim, Sergey Stolyar, and Kristina Hillesland -- Therapeutic microbes for infectious disease / Choon Kit Wong [and others]-- Quantitative measurement and analysis in a synthetic pattern formation multicellular system / Xiongfei Fu and Wei Huang -- Transcriptome analysis of a microbial coculture in which the cell populations are separated by a membrane / Kazufumi Hosoda [and others] -- Identification of mutations in laboratory-evolved microbes from next-generation sequencing data using breseq / Daniel E. Deatherage and Jeffrey E. Barrick -- 3D-fluorescence in situ hybridization of intact, anaerobic biofilm / Kristen A. Brileya, Laura B. Camilleri, and Matthew W. Fields -- Characterization of living bacterial colonies using nanospray desorption electrospray ionization mass spectrometry / Brandi S. Heath, Matthew J. Marshall, and Julia Laskin -- Modeling community population dynamics with the open-source language R / Robin Green and Wenying Shou -- Simulating microbial community patterning using biocellion / Seunghwa Kang, Simon Kahan, and Babak Momeni |
| Summary |
Annotation Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Engineering Multicellular Systems: Methods and Protocols provide a comprehensive laboratory protocol reference for constructing multicellular systems for various applications |
| Analysis |
chemie |
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chemistry |
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biotechnologie |
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biotechnology |
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engineering |
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biochemie |
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biochemistry |
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Chemistry (General) |
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Chemie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 6, 2014) |
| Subject |
Synthetic biology.
|
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Bioengineering.
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Bioengineering -- methods
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Synthetic Biology -- methods
|
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Microbial Consortia
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Models, Molecular
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Synthetic Biology
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Biotechnology
|
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bioengineering.
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Bioengineering
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Synthetic biology
|
| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Sun, Lianhong, editor.
|
|
Shou, Wenying, editor.
|
| ISBN |
9781493905546 |
|
1493905546 |
|
1493905538 |
|
9781493905539 |
|
