Title PCR/RT-PCR in situ light and electron microscopy / Gérard Morel, Mireille Raccurt

Published Boca Raton : CRC Press, ©2003

Click on the following:
Taylor & Francis eBooks
BIOSCIENCEnetBASE

Copies

Description 1 online resource (xvi, 416 pages, 11 unnumbered pages of plates) : illustrations (some color) Series Methods in visualization Methods in visualization. Contents GENERAL PRINCIPLES; PREPARATION OF SAMPLES; PRETREATMENTS; REVERSE TRANSCRIPTION (RT); POLYMERASE CHAIN REACTION (PCR); HYBRIDIZATION; REVELATION; ELECTRON MICROSCOPY; CONTROLS AND PROBLEMS; TYPICAL PROTOCOLS; EXAMPLES OF OBSERVATIONS; APPENDICES; A-Equipment; B-Reagents; GLOSSARY; INDEX; ; Short TOC; General Principles. Preparation of Samples. Pretreatments. Reverse Transcription (RT). Polymerase Chain Reaction (PCR). Hybridization. Revelation. Electron Microscopy. Controls and Problems. Typical Protocols. Examples of Observations. Appendices. Glossary. Index Summary "PCR/RT-PCR in situ Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopic levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers, and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols."--Jacket Notes English Print version record Subject Microscopy -- Technique. Electron microscopy -- Technique. Polymerase chain reaction. Reverse transcriptase. Microscopy -- methods DNA-Directed DNA Polymerase Microscopy, Electron -- methods Polymerase Chain Reaction RNA-Directed DNA Polymerase NATURE -- Reference. SCIENCE -- Life Sciences -- General. SCIENCE -- Life Sciences -- Biology. Electron microscopy -- Technique Microscopy -- Technique Polymerase chain reaction Reverse transcriptase Labortechnik Polymerase-Kettenreaktion In situ Microscópio eletrônico (técnicas) Reação em cadeia por polimerase. Dna. Form Electronic book Author Raccurt, Mireille LC no. 2002073649 ISBN 142004253X 9781420042535 9780429118241 0429118244 1280482613 9781280482618 9786610482610 6610482616

  Permalink