Title Protocols in molecular parasitology / edited by John E. Hyde

Published Totowa, N.J. : Humana Press, ©1993

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Description 1 online resource (xiv, 470 pages) : illustrations Series Methods in molecular biology ; v. 21 Methods in molecular biology (Clifton, N.J.) ; v. 21 Contents Culturing and biological cloning of Trypanosoma brucei -- Culturing and biological cloning of Trypanosoma cruzi -- In vitro cultivation and biological cloning of Leishmania -- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations -- Synchronization and cloning of malaria parasites -- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies -- Culture of Schistosoma mansoni and production of life cycle stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum -- Extraction and purification of nucleic acids from schistosomes -- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting -- Development and use of repetitive sequences as DNA probes for parasite detection and species identification -- Analysis of the DNA of parasitic protozoa by flow cytometry -- PCR for low-level detection of malaria parasites in blood -- PCR methods for identification of point mutations and gene rearrangements -- Antigenic typing of field isolates of Plasmodium by DNA techniques -- Ribosomal RNA probes for detection and identification of species -- Rapid sequencing of parasite rRNA -- Antibody screening of expression libraries -- Antibody select procedure for characterization of expression clones -- Isolation of parasite genes using synthetic oligonucleotides -- Chromosome mapping methods for parasitic protozoa -- Transfection of Leishmania and Trypanosoma brucei by electroporation -- Two-dimensional polyacrylamide gel electrophoresis -- Isoenzyme electrophoresis for parasite characterization -- Surface labeling of schistosomes -- Monclonal antibody affinity chromotography -- Immunoblotting and enzyme linked immunosorbent assay -- Immunofluorescence of parasites -- Immunoelectron microscopic localization of antigens in malaria parasites -- Preparation of blotted membrane for protein microsequencing -- Identification of T-cell epitopes Summary Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation Analysis parasitologie parasitology parasitosen parasitoses parasieten parasites moleculaire biologie molecular biology moleculaire genetica molecular genetics technieken techniques methodologie methodology protocollen protocols Biological Techniques Medicine (General) Medical Entomology Biologische technieken Geneeskunde (algemeen) Medische entomologie Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Molecular parasitology -- Congresses Parasites. Molecular Biology Parasites -- isolation & purification Parasites Parasitology -- methods Parasites Molecular parasitology Genre/Form proceedings (reports) Conference papers and proceedings Laboratory manuals. Conference papers and proceedings. Manuels de laboratoire. Actes de congrès. Form Electronic book Author Hyde, John E. ISBN 9781592595082 1592595081

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