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Title YAC protocols / edited by Alasdair MacKenzie
Edition 2nd ed
Published Totowa, N.J. : Humana Press, ©2006


Description 1 online resource (xv, 203 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; 349
Methods in molecular biology (Clifton, N.J.) ; v. 349
Contents Markers, selection, and media in yeast artificial chromosomes cloning / David Markie -- Construction of yeast artificial chromosomes libraries from pathogens and nonmodel organisms / Cecilia P. Sanchez and Michael Lanzer -- Analysis and screening of yeast artificial chromosomes libraries by filter hybridization / Cecilia P. Sanchez and Michael Lanzer -- Analysis of yeast artificial chromosome DNA by restriction digestion, southern blotting nucleic acid hybridization and polymerase chain reaction / Sylvia Vasiliou and John Peter Quinn -- Postgenomic bioinformatic analysis of yeast artificial chromosomes sequence / Kerry Miller, Scott Davidson, and Alasdair MacKenzie -- Transformation of yeast using bio-active beads with surface-immobilized yeast artificial chromosomes / Shigeki Kawakami [and others]
Modification and amplification of yeast artificial chromosomes / Sanbing Shen, Anthony Harmar, and Nick Hastie -- Modifying yeast artificial chromosomes to generate Cre/LoxP and FLP/FRT site-specific deletions and inversions / Gabriela G. Loots -- Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes / Natalay Kouprina, Vladimir N. Noskov, and Vladimir Larionov -- A polymerase chain reaction-mediated yeast artificial chromosomes-splitting technology for generating targeted yeast artificial chromosomes subclones / Yeon-Hee Kim [and others] -- Genomic reconstruction by serial mitotic recombination of yeast artificial chromosomes / David Markie, Emma Jones, and Jiannis Ragoussis -- Targeting vector construction by yeast artificial chromosome modification / Peter J. Murray
Production of yeast artificial chromosomes transgenic mice by pronuclear injection of one-cell embryos / Alasdair MacKenzie -- Generation of yeast artificial chromosomes transgenic mice by intracytoplasmic sperm injection / Pedro N. Moreira [and others] -- Establishment of cell lines that exhibit correct ontogenic stage-specific gene expression pprofiles from tissues of yeast artificial chromosome transgenic mice using chemically induced growth signals / C. Anthony Blau and Kenneth R. Peterson -- Application of yeast artificial chromosomes in fluorescence in situ hybridization / Thomas Liehr -- Contribution of yeast artificial chromosome-based physical maps to the final assembly of the trypanosoma cruzi genome / Márcia R.M. Santos [and others]
Summary It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Molecular cloning -- Methods
Genetic vectors.
Yeast fungi -- Genetic engineering.
Saccharomyces cerevisiae.
Genetics -- Technique.
Artificial chromosomes.
Chromosomes, Artificial, Yeast
Genetic Vectors
Genetic Techniques
Chromosomes, Artificial
Genetic Structures
Chromosomes, Fungal
Investigative Techniques
Fungal Structures
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Genetic Phenomena
Cellular Structures
Phenomena and Processes
Saccharomyces cerevisiae
Animal Structures
SCIENCE -- Biotechnology.
Genetics -- Technique
Artificial chromosomes
Genetic vectors
Molecular cloning
Saccharomyces cerevisiae
Yeast fungi -- Genetic engineering
Genre/Form Laboratory manuals
Methods (Music)
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author MacKenzie, Alasdair.
ISBN 1588296121