Title Chaperonin protocols / edited by Christine Schneider

Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (x, 212 pages) : illustrations Series Methods in molecular biology ; v. 140 Methods in molecular biology (Clifton, N.J.) ; v. 140 Contents Purification of Archaeal Chaperonin from Sulfolobus shibatae / Elsie Quaite-Randall, Andrzej Joachimiak -- Purification of Hsp from Thermus thermophilus / Elsie Quaite-Randall, Andrzej Joachimiak -- Purification of GroEL from an Overproducing E. coli Strain / Elsie Quaite-Randall, Andrzej Joachimiak -- Purification of GroES from an Overproducing E. coli Strain / Elsie Quaite-Randall, Andrzej Joachimiak -- uPirifacitnoo fht epGC -ohcparenonif or maBtcreoihpga eT / Saskia M. van der Vies -- Removing Trace Fluorescent Contaminants from GroEL Preparations / Frank Weber -- Assembly and Disassembly of GroEL and GroES Complexes / Jorg Martin -- GroEL/GroES Interaction Assayed by Protease Protection / Jorg Martin -- Determination of Chaperonin Activity In Vivo / Saskia M. van der Vies, Peter A. Lund -- Interaction of Nonnative Polypeptide Substrates with the Escherichia coli Chaperonin GroEL / Wanda K. Hartmann, Edward Eisenstein -- Prevention of Rhodanese Aggregation by the Chaperonin GroEL / Frank Weber, Manajit Hayer-Hartl -- Refolding of Bovine Mitochondrial Rhodanese by Chaperonins GroEL and GroES / Frank Weber, Manajit Hayer-Hartl -- Assay of Malate Dehydrogenase : A Substrate for the E. coli Chaperonins GroEL and GroES / Manajit Hayer-Hartl -- Assay of Chaperonin-Assisted Refolding of Citrate Synthase / N. Kalaya Steede, Stacy L. Temkin, Samuel J. Landry -- Purification of Yeast Mitochondrial Hsp / Yves Dubaquie, Renate Looser, Sabine Rospert -- Preparation of Recombinant Human Hsp / N. Kalaya Steede, Samuel J. Landry, Jesse J. Guidry -- Purification of the Cytosolic Chaperonin TRiC from Bovine Testis / Raul G. Ferreyra, Judith Frydman -- Monitoring Actin Folding : Purification Protocols for Labeled Proteins and Binding to DNase I-Sepharose Beads / Raul G. Ferreyra, Judith Frydman, Vanitha Thulasiraman -- Folding Assays : Assessing the Native Conformation of Proteins / Raul G. Ferreyra, Judith Frydman, Vanitha Thulasiraman -- Purification of Prefoldin / Sally A. Lewis, Nicholas J. Cowan -- Purification of GimC from Saccharomyces cerevisiae / Katja Siegers, Elmar Schiebel -- Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding / Michel R. Leroux Summary "In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods--for example, one for preparing labeled probes--that can be used for various purposes and prove helpful in numerous different procedures." "Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results. Book jacket."--Jacket Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Molecular chaperones -- Purification -- Laboratory manuals Chaperonins -- analysis Chaperonins -- isolation & purification SCIENCE -- Life Sciences -- Biochemistry. Biochemistry Life sciences Molecular biology Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Schneider, Christine, 1961- LC no. 99041622 ISBN 9781592590612 0896037398 9780896037397 0585330743 9780585330747 1592590616 1280820527 9781280820526 9786610820528 661082052X

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