Title Cyclic nucleotide signaling in plants : methods and protocols / edited by Chris Gehring

Published New York : Humana Press, ©2013

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Description 1 online resource (x, 273 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 1016 Methods in molecular biology (Clifton, N.J.) ; v. 1016 Contents Molecular methods for the study of signal transduction in plants / Helen R. Irving and Chris Gehring -- Recombinant expression and functional testing of candidate adenylate cyclase domains / Oziniel Ruzvidzo [and others] -- Quantification of cyclic dinucleotides by reversed-phase LC-MS/MS / Heike Burhenne and Volkhard Kaever -- Determination of ADP-ribosyl cyclase activity, cyclic ADP-ribose, and nicotinic acid adenine dinucleotide phosphate in tissue extracts / Richard M. Graeff and Hon Cheung Lee -- In vivo imaging of cGMP in plants / Jean-Charles Isner and Frans J.M. Maathuis -- Characterization of heterologously expressed transporter genes by patch- and voltage-clamp methods : application to cyclic nucleotide-dependent responses / Fouad Lemtiri-Chlieh and Rashid Ali -- Noninvasive microelectrode ion flux estimation technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides / Natalia Maria Ordoñez [and others] -- Calcium imaging of the cyclic nucleotide response / Martin R. McAinsh, Stephen K. Roberts, and Lyudmila V. Dubovskaya -- Identification and quantitation of signal molecule-dependent protein phosphorylation / Arnoud Groen [and others] -- Comparative gel-based phosphoproteomics in tesponse to signaling molecules / Claudius Marondedze, Kathryn Lilley, and Ludivine Thomas -- Affinity pull-down approach to identify the plant cyclic nucleotide interactome / Lara Donaldson and Stuart Meier -- Structural and functional characterization of receptor kinases with nucleotide cyclase activity / Victor Muleya, Janet I. Wheeler, and Helen R. Irving -- Computational identification of candidate nucleotide cyclases in higher plants / Aloysius Wong and Chris Gehring -- Identification of cyclic nucleotide gated channels using regular expressions / Alice K. Zelman, Adam Dawe, and Gerald A. Berkowitz -- Inferring biological functions of guanylyl cyclases with computational methods / May Alqurashi and Stuart Meier -- Identification and characterization of cyclic nucleotide phosphodiesterases / Erin B. Purcell and Rita Tamayo -- Detection of reactive oxygen species downstream of cyclic nucleotide signals in plants / Robin K. Walker and Gerald A. Berkowitz -- Measurement of nitric oxide in plant tissue using difluorofluorescein and oxyhemoglobin / Ndiko Ludidi -- Infrared gas analysis technique for the study of the regulation of photosynthetic responses / Alex Valentine [and others] Summary Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling Analysis Life sciences Biochemistry Botany Plant Sciences Plant Biochemistry plantkunde signaaltransductie signal transduction plantensamenstelling plant composition biochemie plantencelbiologie plant cell biology Plant Cell Biology Plantencelbiologie Bibliography Includes bibliographical references and index Notes English Subject Cyclic nucleotides -- Laboratory manuals Plant cellular signal transduction -- Laboratory manuals Cell physiology. Life sciences. Nucleotides. Physical sciences. Cellular signal transduction. Cyclic nucleotides. Physiology. Publication Formats Biochemical Phenomena Cell Physiological Phenomena Biological Science Disciplines Nucleotides Natural Science Disciplines Nucleic Acids, Nucleotides, and Nucleosides Chemical Phenomena Publication Characteristics Phenomena and Processes Chemicals and Drugs Disciplines and Occupations Signal Transduction Nucleotides, Cyclic Physiology Laboratory Manuals biological sciences. physical sciences. physiology. Physiology Physical sciences Nucleotides Life sciences Cellular signal transduction Cell physiology Cyclic nucleotides Plant cellular signal transduction Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Gehring, Christoph. ISBN 9781627034418 1627034412

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