Title Guanylate cyclase and cyclic GMP : methods and protocols / edited by Thomas Krieg, Robert Lukowski

Published New York : Humana Press, [2013] ©2013

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Description 1 online resource (xi, 249 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 1020 Methods in molecular biology (Clifton, N.J.) ; v. 1020. 1064-3745 Contents NO/cGMP: The past, the present, and the future -- cGMP-dependent protein kinases (cGK) -- Enzyme assays for cGMP hydrolyzing phosphodiesterases -- Radioimmunoassay for the quantification of cGMP levels in cells and tissues -- Hyperspectral imaging of FRET-based cGMP probes -- Visualization of cGMP with cGi biosensors -- Advances and techniques to measure cGMP in intact cardiomyocytes -- Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells -- Methods for identification of cGKI substrates -- Approaches for monitoring PKG1alpha oxidative activation -- Analysis of cGMP signaling in adipocytes -- A genetic strategy for the analysis of individual axon morphologies in cGMP signalling mutant mice -- Receptor binding assay for no-independent activators of soluble guanylate cyclase -- Direct intrathecal drug delivery in mice for detecting in vivo effects of cGMP on pain processing -- The Geisler method: Tracing activity-dependent cGMP plasticity changes upon double detection of mRNA and protein on brain slices -- Detection of cGMP in the degenerating retina Summary Recently, many ground-breaking steps have been made towards better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide scientist current methods and a useful guide towards the possibility to apply these techniques to their own research Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (Springer, viewed May 29, 2013) Subject Guanylate cyclase. Cyclic guanylic acid. Cyclic nucleotides. Lyases. Purine nucleotides. Nucleotides. Proteins. Enzymes. Alloxuric substances. Purines. Heterocyclic compounds. Publication Formats Phosphorus-Oxygen Lyases Nucleotides, Cyclic Intracellular Signaling Peptides and Proteins Guanine Nucleotides Lyases Purine Nucleotides Publication Characteristics Peptides Ribonucleotides Nucleotides Proteins Enzymes Amino Acids, Peptides, and Proteins Purines Nucleic Acids, Nucleotides, and Nucleosides Chemicals and Drugs Enzymes and Coenzymes Heterocyclic Compounds, 2-Ring Heterocyclic Compounds Cyclic GMP Guanylate Cyclase Laboratory Manuals protein. enzyme. Purines Purine nucleotides Proteins Nucleotides Lyases Heterocyclic compounds Enzymes Cyclic nucleotides Alloxuric substances Cyclic guanylic acid Guanylate cyclase Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Krieg, Thomas, 1967- editor Lukowski, Robert, 1977- editor ISBN 9781627034593 1627034595

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