Title Tandem repeats in genes, proteins, and disease : methods and protocols / edited by Danny M. Hatters, Anthony J. Hannan

Published New York : Humana Press, ©2013

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Description 1 online resource (xi, 258 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; 1017 Methods in molecular biology (Clifton, N.J.) ; v. 1017. 1064-3745 Contents Longitudinal imaging and analysis of neurons expressing polyglutamine-expanded proteins / Andrey S. Tsvetkov, D. Michael Ando, and Steven Finkbeiner -- Atomic force microscopy assays for evaluating polyglutamine aggregation in solution and on surfaces / Kathleen A. Burke and Justin Legleiter -- Morphometric analysis of Huntington's disease neurodegeneration in Drosophila / Wan Song [and others] -- Size analysis of polyglutamine protein aggregates using fluorescence detection in an analytical ultracentrifuge / Saskia Polling, Danny M. Hatters, and Yee-Foong Mok -- Method for the incremental expansion of polyglutamine repeats in recombinant proteins / Amy L. Robertson and Stephen P. Bottomley -- Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry : applications for polyglutamine aggregation / Yasmin M. Ramdzan, Rebecca Wood, and Danny M. Hatters -- Characterizing social behavior in genetically targeted mouse models of brain disorders / Emma L. Burrows and Anthony J. Hannan -- PCR amplification and sequence analysis of GC-rich sequences : aristaless-related homeobox example / May H. Tan, Jozef Gécz, and Cheryl Shoubridge -- Challenges of "Sticky" co-immunoprecipitation : polyalanine tract protein-protein interactions / T.R. Mattiske [and others] -- Molecular pathology of polyalanine expansion disorders : new perspectives from mouse models / James N. Hughes and Paul Q. Thomas -- Yeast as a platform to explore polyglutamine toxicity and aggregation / Martin L. Duennwald -- Immuno-based detection assays to quantify distinct mutant huntingtin conformations in biological samples / Gregor P. Lotz and Andreas Weiss -- Modeling and analysis of repeat RNA toxicity in Drosophila / S.E. Samaraweera [and others] -- Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis / Mats Holmberg and Ellen A.A. Nollen -- Kinetic analysis of aggregation data / Regina M. Murphy -- Bioinformatics method for identifying Q/N-rich prion-like domains in proteins / Eric D. Ross [and others] -- Detecting soluble polyQ oligomers and investigating their impact on living cells using split-GFP / Patrick Lajoie and Erik Lee Snapp -- Cell biological approaches to investigate polyglutamine-expanded AR metabolism / Lori J. Cooper and Diane E. Merry Summary The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences Analysis Medicine Human genetics Medical genetics Biomedicine Gene Function biomedische wetenschappen moleculaire genetica molecular genetics humane genetica Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Subject Nucleotides -- Laboratory manuals Genetics -- Laboratory manuals Biology. Genomes. Life sciences. Physical sciences. Molecular structure. Diseases. Publication Formats Repetitive Sequences, Nucleic Acid Biology Genome Components Pathologic Processes Phenomena and Processes Genome Pathological Conditions, Signs and Symptoms Base Sequence Publication Characteristics Biological Science Disciplines Natural Science Disciplines Genetic Structures Molecular Structure Disease Disciplines and Occupations Biochemical Phenomena Chemical Phenomena Genetic Phenomena Tandem Repeat Sequences Genetics Laboratory Manuals biology. biological sciences. physical sciences. molecular structure. disease. illness. Physical sciences Molecular structure Life sciences Genomes Diseases Biology Genetics Nucleotides Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hatters, Danny M Hannan, Anthony J., 1969- ISBN 9781627034388 1627034382 1627034374 9781627034371

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