| Description |
1 online resource (x, 274 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 169 |
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Methods in molecular biology (Clifton, N.J.) ; v. 169
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| Contents |
Localization of neurotrophin proteins within the central nervous system by immunohistochemistry / J.M. Conner -- Neurotrophin immunohistochemistry in peripheral tissues / R.A. Rush and X.F. Zhou -- Extraction and quantification of the neurotrophins / S.H. Zhang, X.F. Zhou and R.A. Rush -- Identifying novel proteins in nervous tissue using microsequencing techniques / S.E. Bartlett -- Quantification of mRNA levels using northern blotting / P. Fernyhough -- Quantification of mRNA levels using ribonuclease protection assay / P. Henttu -- Quantification of neurotrophin mRNA by RT-PCR / E. Chie [and others] -- Sensitive and nonradioactive in situ detection of neurotrophin mRNAs in the nervous system / C.J. Xian and X.F. Zhou -- Expression of neurotrophic factors such as GDNF in the E. Coli system / B.R. Huang and X.M. Ma -- Construction of cells expressing neurotrophins / J. Ray and F.H. Gage -- Efficient generation of stable pheochromocytoma (PC12) cell lines using a recombinant retrovirus (lnc) / M.E. Cunningham, J.K. Kitajewski and L.A. Greene -- Construction and analysis of transgenic animals overexpressing neurotrophins / K.M. Albers and B.M. Davis -- Construction of transgenic animals overproducing neurotrophins and their receptors / C.G. Causing and F.D. Miller -- Tracing with radiolabeled neurotrophins / C.S. von Bartheld -- Toxin-induced death of neurotrophin-sensitive neurons / R.G. Wiley -- Oligonucleotides as inhibitors of protein synthesis / W.R. Epa, G.L. Barrett and P.F. Bartlett -- The IODO-GEN method for labeling and the use of retrograde axonal transport to assay neurotrophins / I.A. Hendry and A.J. Reynolds -- Stereological estimation of neuronal number. The optical disector/cavalieri combination / V. Nurcombe, N.G. Wreford and J.F. Bertram |
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Neurotrophin quantification and localization -- RNA quantification and localization -- Construction of recombinant proteins, and transgenic cells and animals -- In vivo analysis |
| Summary |
Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| In |
Springer Protocols |
| Subject |
Nerve growth factor -- Laboratory manuals
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Nerve Growth Factors
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SOCIAL SCIENCE -- Anthropology -- Physical.
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Nerve growth factor
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Wetenschappelijke technieken.
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Rush, Robert A.
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| LC no. |
00044824 |
| ISBN |
0896036995 |
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9780896036994 |
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9781592590605 |
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1592590608 |
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1280820519 |
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9781280820519 |
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9786610820511 |
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6610820511 |
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