| Description |
1 online resource (xiii, 386 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 73 |
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Methods in molecular biology (Clifton, N.J.) ; volume 73
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| Contents |
Preparation of neuropeptide-containing fractions from biological materials / J. Michael Conlon -- Purification of extracted peptides for structural analysis / Chris Shaw -- Amino acid sequencing of neuropeptides / Ka Wan Li, Wijnand P. Geraerts -- Neuropeptide gene identification using the polymerase chain reaction / Aaron G. Maule, Timothy G. Geary -- Solid-phase synthesis of neuropeptides by fmoc strategies / Chris Kowalczyk, Michael O'Shea -- Incorporation of stable pseudopeptide bonds : methylene amino, thioether, and hydroxyethylene derivatives / Graeme J. Anderson -- Synthesis of conformationally restricted peptides / Annette G. Beck-Sickinger -- Purification of synthetic peptides by high performance liquid chromatography / D. David Smith, Ann M. Hanly -- Molecular weight estimation for neuropeptides using size-exclusion high performance liquid chromatography / G. Brent Irvine -- Molecular weight determinations using polyacrylamide gel electrophoresis with tris-tricine buffers / G. Brian Wisdom -- Determination of neuropeptides by capillary electrophoresis / SungAe S. Park, Wei-Lun Hung, Daniel E. Schaufelberger, Norberto A. Guzman, Juan P. Advis -- Characterization of neuropeptide processing by fast atom bombardment mass spectrometry / Jerzy Silberring -- Analysis of neuropeptides by size-exclusion HPLC linked to electrospray ionization mass spectrometry / Jerzy Silberring -- Identification of peptides by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) and direct analysis of the laterobuccal nerve from the pond snail lymnaea stagnalis / Glenn Critchley, Belinda Worster -- Use of circular dichroism to determine secondary structure of neuropeptides / Laszlo Otvos -- ¹H nuclear magnetic resonance (NMR) in the elucidation of peptide structure / David J. Guthrie -- The study of membrane- or receptor-bound neuropeptides by NMR / Rickey P. Hicks -- Molecular modeling of neuropeptides / Sándor Lovas, Richard F. Murphy -- Tritium labeling of neuropeptides / Géza Tóth, Sándor Lovas, Ferenc Ötvös -- The use of IODO-GEN for preparing ¹²⁵I-labeled peptides and their purification by reversed-phase high performance liquid chromatography / J. Michael Conlon -- Production of antisera using peptide conjugates / Thomas E. Adrian -- Radioimmunoassay / Thomas E. Adrian -- Enzyme-linked immunosorbent assay of peptides / Laszlo Otvos, Gyorgyi I. Szendrei -- Sample preparation for peptide immunocytochemistry / Kathy M. Pogue, Colin F. Johnston -- Immunocytochemical methods for regulatory peptides / Kathy M. Pogue, Colin F. Johnston -- Ultrastructural localization of peptides using immunogold labeling / David W. Halton, Gerard P. Brennan -- Preparation of a membrane fraction for receptor studies and solubilization of receptor proteins with retention of biological activity / Mark Wheatley, John Howl, Nicola J. Yarwood, Andrew R. Davies, Rosemary A. Parslow -- Radioligand binding using ¹²⁵I-labeled peptides / Peter W. Abel, David Waugh, William B. Jeffries -- Analysis of data from "cold saturation" radioligand binding experiments / William B. Jeffries, David Waugh, Peter W. Abel -- Organ/tissue preparations for the assessment of agonist/antagonist activity / Mark Murnin, Sándor Lovas, James M. Allen, Richard F. Murphy -- Measurement of efflux rates from brain to blood / William A. Banks, Melita B. Fasold, Abba J. Kastin -- Assay of neuropeptidases using fluorogenic substrates / Carvell H. Williams -- Characterization of neuropeptidases using inhibitors / Nigel M. Hooper |
| Summary |
Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols |
| Analysis |
biomedische wetenschappen |
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biomedicine |
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neurowetenschap |
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neuroscience |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Neuropeptides -- Analysis -- Laboratory manuals
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Neuropeptides -- analysis
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Neuropeptides -- chemical synthesis
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Neuropeptides -- Analysis
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Diagnostic par imagerie -- Méthode.
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Neuropeptiden.
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Irvine, G. Brent.
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Williams, Carvell H.
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| ISBN |
9781592595594 |
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1592595596 |
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1280836792 |
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9781280836794 |
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9786610836796 |
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6610836795 |
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