Title Coronaviruses : methods and protocols / edited by Helena Jane Maier, Erica Bickerton, Paul Britton

Published New York : Humana Press : Springer, ©2015

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Description 1 online resource (xi, 285 pages) : color illustrations Series Methods in molecular biology, 1940-6029 ; 1282 Methods in molecular biology (Clifton, N.J.) ; v. 1282. 1064-3745 Contents Coronaviruses : an overview of their replication and pathogenesis / Anthony R. Fehr and Stanley Perlman -- Identification of a novel coronavirus from Guinea Fowl using metagenomics / Mariette F. Ducatez and Jean-Luc Guérin -- Serological diagnosis of feline coronavirus infection by immunochromatographic test / Tomomi Takano and Tsutomu Hohdatsu -- Estimation of evolutionary dynamics and selection pressure in coronaviruses / Muhammad Munir and Martí Cortey -- Preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration / Ruth M. Hennion -- Preparation of chicken kidney cell cultures for virus propagation / Ruth M. Hennion and Gillian Hill -- Isolation and propagation of coronaviruses in embryonated eggs / James S. Guy -- Characterization of human coronaviruses on well- differentiated human airway epithelial cell cultures / Hulda R. Jonsdottir and Ronald Dijkman -- Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo / Joeri Kint, Helena Jane Maier, and Erik Jagt -- Purification of coronavirus virions for Cryo-EM and proteomic analysis / Stuart Dent and Benjamin W. Neuman -- Partial purification of IBV and subsequent isolation of viral RNA for next-generation sequencing / Sarah M. Keep, Erica Bickerton, and Paul Britton -- Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses / Sarah M. Keep, Erica Bickerton, and Paul Britton -- Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes / Fernando Almazán [and others] -- Protein histochemistry using coronaviral spike proteins : studying binding profiles and sialic acid requirements for attachment to tissues / Iresha N. Ambepitiya Wickramasinghe and M. Hélène Verheije -- Identification of protein receptors for coronaviruses by mass spectrometry / V. Stalin Raj [and others] -- Single particle tracking assay to study coronavirus membrane fusion / Deirdre A. Costello and Susan Daniel -- Studying coronavirus-host protein interactions / Chee-Hing Yang [and others] -- Field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions / Pierre- Olivier Vidalain [and others] -- Investigation of the functional roles of host cell proteins involved in coronavirus infection using highly specific and scalable RNA interference (RNAi) approach / Jean Kaoru Millet and Béatrice Nal -- Transcriptome analysis of feline infectious peritonitis virus infection / Parvaneh Mehrbod [and others] -- Quantification of interferon signaling in avian cells / Joeri Kint and Maria Forlenza -- Studying the dynamics of coronavirus replicative structures / Marne C. Hagemeijer and Cornelis A.M. de Haan -- Preparation of cultured cells using high-pressure freezing and freeze substitution for subsequent 2D or 3D visualization in the transmission electron microscope / Philippa C. Hawes Summary This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields Analysis virologie virology microbiologie microbiology coronavirus Virology Virologie Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed March 3, 2015) Subject Coronaviruses -- Laboratory manuals Coronaviruses. Nidoviruses. RNA viruses. Viruses. Coronaviridae Publication Formats Nidovirales Publication Characteristics RNA Viruses Viruses Vertebrate Viruses Organisms Coronavirus Laboratory Manuals Medical microbiology & virology. Microbiology (non-medical) Medical -- Microbiology. Science -- Life Sciences -- Biology -- Microbiology. Viruses RNA viruses Nidoviruses Coronaviruses Genre/Form handbooks. manuals (instructional materials) Laboratory manuals Handbooks and manuals Laboratory manuals. Handbooks and manuals. Manuels de laboratoire. Guides et manuels. Form Electronic book Author Maier, Helena Jane, editor. Bickerton, Erica, editor. Britton, Paul W., editor. ISBN 9781493924387 1493924389 1493924370 9781493924370

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