Title Capillary electrophoresis of nucleic acids. Volume 162, Part 1, Introduction to the capillary electorphoresis of nucleic acids / edited by Keith R. Mitchelson and Jing Cheng

Published Totowa, N.J. : Humana Press, ©2001

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Description 1 online resource (1 volume) : illustrations Series Methods in molecular biology ; v. 162, pt. 1 Methods in molecular biology (Clifton, N.J.) ; volume 162, pt. 1 Contents The application of capillary electrophoresis for DNA polymorphism analysis / K.R. Mitchelson -- DNA separation mechanisms during electrophoresis / G.W. Slater, C. Desruisseaux and S.J. Hubert -- Purification methods for preparing polymerase chain reaction products for capillary electrophoresis analysis / J.M. Devaney and M.A. Marino -- Analytical considerations for capillary electrophoresis. Quantitative measurements / B. McCord -- Microchip-based capillary electrophoresis systems / W. Xie [and others] -- DNA sequencing by capillary array electrophoresis / N.J. Dovichi and J. Zhang -- Determining dye-induced DNA mobility shifts for DNA sequencing fragments by capillary electrophoresis / J. Bashkin -- Influence of polymer concentration and polymer composition on capillary electrophoresis of DNA / C. Heller -- Capillary coatings. Choices for capillary electrophoresis of DNA / M. Chiari and M. Cretich -- Replaceable polymers for DNA sequencing by capillary electrophoresis / M.A. Quesada and S. Menchen -- Sieving matrix selection / T. Wehr, M. Zhu and D.T. Mao -- Parameters affecting capillary electrophoretic separation of DNA / H.J. Issaq -- DNA separation by capillary electrophoresis in lyotropic polymer liquid crystals / Y. Liu and R.L. Rill -- Capillary electrophoresis of DNA fragments using poly(ethylene oxide) as a sieving material / Y. Kim and E.S. Yeung -- DNA capillary electrophoresis using block copolymer as a new separation medium / B. Chu [and others] -- DNA analysis under highly denaturing conditions in bare fused silica capillaries / K. Kleparnik, Z. Mala and P. Bocek -- Capillary electrophoresis with glycerol as an additive / K.R. Mitchelson and J. Cheng -- Capillary electrophoresis of DNA fragments with replaceable low-gelling agarose gels / A.K. Palm -- Robust field inversion capillary electrophoretic separation of long DNA fragments / C. Heller, S. Magnusdottir and J.L. Viovy -- Pulsed-field capillary electrophoresis separation of large DNA fragments / M.D. Morris, J.J. Schwinefus and O. de Carmejane -- Collection of capillary electrophoresis fractions on a moving membrane / S. Magnusdottir [and others] -- Separation of supercoiled DNA using capillary electrophoresis / D.T. Mao and R.M. Lautamo -- Quality control of nucleotides and primers for pcr / M.J. Pearce and N.D. Watson -- Analysis of modified oligonucleotides with capillary gel electrophoresis / L.A. DeDionisio -- Use of capillary electrophoresis for concentration analysis of phosphorothioate oligonucleotides / G.S. Srivatsa, R. Pourmand and S. Winters -- Capillary electrophoresis separation of ribonucleosides / D.K. Xu and H.Y. Chen -- On-line preconcentration and separation of antisense oligonucleotides by itp-ce in dextran-filled capillaries / I. Barme and G.J. Bruin -- Capillary electrophoresis-mass spectrometric analysis of DNA adducts / L.A. Marzilli, C. Koertje and P. Vouros -- Analysis of environment-induced DNA damage by capillary electrophoresis / Z.E. Nackerdien [and others] -- Assay for DNA damage using immunochemical recognition and capillary electrophoresis / J.Z. Xing [and others] -- Analysis of DNA damage using capillary zone electrophoresis and electrospray mass spectrometry / D.L. Deforce and E.G. Van den Eeckhout -- Integration of phosphodiesterase-induced degradation of oligonucleotides with capillary polymer-sieving electrophoresis / J. Saevels, A. Van Schepdael and J. Hoogmartens -- Capillary electrophoresis of urinary normal and modified nucleosides of cancer patients / G. Xu [and others] Summary The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This first volume of the set, Introduction to the Capillary Electrophoresis of Nucleic Acids, covers the practical and theoretical considerations behind the use of capillary electrophoresis for the analysis of small oligonucleotides and modified nucleotides. Along with detailed instructions ensuring ready reproducibility, these protocols offer time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE. Several chapters are devoted to the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides by CE. The companion volume, Practical Applications of Capillary Electrophoresis, addresses techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions. Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today Analysis nucleic acids capillaire electroforese capillary electrophoresis Nucleic Acids Analytical Chemistry Analytische chemie Bibliography Includes bibliographical references and index Notes Print version record Subject Nucleic acids -- Separation. Capillary electrophoresis. Electrophoresis, Capillary Nucleic Acids -- analysis Nucleic Acids -- isolation & purification SCIENCE -- Life Sciences -- Molecular Biology. Capillary electrophoresis Nucleic acids -- Separation Capillaire elektroforese. Nucleïnezuren. Wetenschappelijke technieken. Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Mitchelson, Keith R. Cheng, Jing, 1963- ISBN 9781592590551 1592590551

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