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Title Expressed sequence tags (ESTs) : generation and analysis / edited by John Parkinson
Published New York : Humana, ©2009


Description 1 online resource (x, 331 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; v. 533
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 533. 1064-3745
Springer protocols (Series)
Contents Expressed sequence tags : an overview / John Parkinson and Mark Blaxter -- Strategies for undertaking expressed sequence tag (EST) projects / Sandra W. Clifton and Makedonka Mitreva -- Construction of cDNA libraries : focus on protists and fungi / Naiara Rodríguez-Ezpeleta [and others] -- Generation of full-length cDNA libraries : focus on plants / Motoaki Seki [and others] -- Preparation of full-length cDNA libraries : focus on metazoans / Masako Harada and Yoshihide Hayashizaki -- Expressed sequence tags : normalization and subtraction of cDNA libraries / Marcelo Bento Soares [and others] -- Generating EST libraries : trans-spliced cDNAs / Cecilia Fernández and Rick M. Maizels -- Large-scale sequencing and analytical processing of ESTs / Makedonka Mitreva and Elaine R. Mardis -- EST processing : from trace to sequence / Ralf Schmid and Mark Blaxter -- Obtaining accurate translations from expressed sequence tags / James Wasmuth and Mark Blaxter -- EST databases and web tools for EST projects /Yao-Qing Shen [and others] -- Phylogenomic analysis of EST datasets / José M. Peregrín-Alvarez and John Parkinson -- Statistical analysis of expressed sequence tags / Edward Susko and Andrew J. Roger -- Application of ESTs in microarray analysis / Weizhong Li [and others] -- Exploiting ESTs in human health / Sandro José de Souza
Summary Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In Expressed Sequence Tags (ESTs): Generation and Analysis, leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Expressed Sequence Tags (ESTs): Generation and Analysis serves as an ideal reference for scientists continuing the vital investigation of genes and the genome
Bibliography Includes bibliographical references and index
Notes English
In Springer Protocols
Subject Nucleotide sequence -- Methodology
Gene libraries.
Expressed Sequence Tags
Gene Library
Genomic Library
Gene libraries
Nucleotide sequence -- Methodology
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Parkinson, John, 1968-
ISBN 9781588297594